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Monkey Myoglobin ELISA Kit (ab269549)

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SimpleStep ELISA Protocol Diagram
  • Example of monkey Myoglobin standard curve in Sample Diluent NS.
  • Interpolated concentrations of native Myoglobin in monkey plasma - heparin samples.
  • Sandwich ELISA - Monkey Myoglobin ELISA Kit (ab269549)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 933.4 pg/ml
  • Range: 1250 pg/ml - 80000 pg/ml
  • Sample type: Hep Plasma
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Monkey

製品の概要

  • 製品名

    Monkey Myoglobin ELISA Kit
    Myoglobin キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Plasma 8 7.3%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Plasma 3 8.3%

  • サンプルの種類

    Hep Plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    933.4 pg/ml

  • 検出範囲

    1250 pg/ml - 80000 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Hep Plasma 106 96% - 119%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Monkey

  • 製品の概要

    Monkey Myoglobin ELISA Kit (ab269549) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Myoglobin protein in hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey Myoglobin with 933.4 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 特記事項

    Myoglobin, a heme protein is found in both cardiac and skeletal muscle and functions as a reserve supply of oxygen by facilitating the movement of oxygen within muscles. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours.

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

画像

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of monkey Myoglobin standard curve in Sample Diluent NS.
    Example of monkey Myoglobin standard curve in Sample Diluent NS.

    The Myoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Myoglobin in monkey plasma - heparin samples.
    Interpolated concentrations of native Myoglobin in monkey plasma - heparin samples.

    The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 218.62 ng/mL in plasma (heparin).

  • Sandwich ELISA - Monkey Myoglobin ELISA Kit (ab269549)
    Sandwich ELISA - Monkey Myoglobin ELISA Kit (ab269549)
    To learn more about the advantages of recombinant antibodies see here.

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