Monkey Galectin-3 ELISA Kit (LGALS3) (ab269556)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 115 pg/ml
- Range: 208.9 pg/ml - 6000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
製品の概要
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製品名
Monkey Galectin-3 ELISA Kit (LGALS3)
Galectin 3 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 3.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 3 2.6% -
サンプルの種類
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
115 pg/ml -
検出範囲
208.9 pg/ml - 6000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 87 83% - 90% Serum 105 100% - 110% Cell culture media 83 81% - 85% Hep Plasma 109 108% - 110% EDTA Plasma 106 102% - 110% Cit plasma 117 116% - 118% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Monkey -
製品の概要
Monkey Galectin-3 ELISA Kit (LGALS3) (ab269556) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-3 (LGALS3) protein in edta plasma, hep plasma, serum, cell culture media, cit plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey Galectin-3 (LGALS3) with 115 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is a member of the galectin family of carbohydrate-binding proteins. Galectin-3 is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is often pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Monkey Galectin-3 (LGALS3) Capture Antibody 1 x 600µl 10X Monkey Galectin-3 (LGALS3) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Monkey Galectin-3 (LGALS3) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
組織特異性
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
配列類似性
Contains 1 galectin domain. -
細胞内局在
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
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別名
- 35 kDa lectin
- Carbohydrate binding protein 35
- Carbohydrate-binding protein 35
see all
関連製品
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Alternative Versions
画像
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of monkey Galectin-3 standard curve in Sample Diluent NS.The Galectin-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Galectin-3 in Rhesus Macaque monkey serum and plasma samples.The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 4%, plasma (citrate) 4%, plasma (EDTA) 4% and plasma (heparin) 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 93 ng/mL in neat serum, 111 ng/mL in neat plasma (citrate), 93 ng/mL in neat plasma (EDTA) and 88 ng/mL in neat plasma (heparin).
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Interpolated concentrations of native Galectin-3 in Rhesus Macaque monkey PBMC cell culture supernatant cultured for 24 hours.The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 57%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 7.5 ng/mL in PBMC supernatant.
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Sandwich ELISA - Monkey Galectin-3 ELISA Kit (LGALS3) (ab269556)To learn more about the advantages of recombinant antibodies see here.
参考文献 (0)
ab269556 は論文での使用が確認できていません。
