Anti-Moesin 抗体 [MSN491] - BSA and Azide free (ab269735)
![Western blot - Anti-Moesin antibody [MSN491] - BSA and Azide free (ab269735)](https://www.abcam.co.jp/ps/products/269/ab269735/Images/ab269735-454684-AntiMoesin-antibody-westernblot.jpg "Western blot - Anti-Moesin antibody [MSN491] - BSA and Azide free (ab269735)")
Key features and details
- Mouse monoclonal [MSN491] to Moesin - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Moesin antibody [MSN491] - BSA and Azide free
Moesin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MSN491] to Moesin - BSA and Azide free -
由来種
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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免疫原
Recombinant full length protein corresponding to Human Moesin aa 1 to the C-terminus.
Database link: P26038 -
ポジティブ･コントロール
- HT29, CH3LC, or HUVEC cells. Uterus, placenta, tonsil (both B and T lymphocytes), skeletal muscle, thyroid, or kidney; Human placenta tissue, PC3 cell lysate.
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特記事項
ab269735 is a carrier free version of ab193380. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
MSN491 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Cow
Pig
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa.
|
|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa. |
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. -
組織特異性
In all tissues and cultured cells studied. -
配列類似性
Contains 1 FERM domain. -
翻訳後修飾
Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. -
細胞内局在
Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment. - Information by UniProt
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参照データベース
- Entrez Gene: 540426 Cow
- Entrez Gene: 4478 Human
- Entrez Gene: 17698 Mouse
- Entrez Gene: 494458 Pig
- Entrez Gene: 81521 Rat
- Omim: 309845 Human
- SwissProt: Q2HJ49 Cow
- SwissProt: P26038 Human
see all -
別名
- Epididymis luminal protein 70 antibody
- HEL70 antibody
- Membrane organizing extension spike protein antibody
see all
画像
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Western blot - Anti-Moesin antibody [MSN491] - BSA and Azide free (ab269735)Anti-Moesin antibody [MSN491] (ab193380) at 2 µg/ml + human PC3 cell lysate
Predicted band size: 68 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody (ab269735)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab269735 は論文での使用が確認できていません。