Anti-MMP3 抗体 (ab53015)
Key features and details
- Rabbit polyclonal to MMP3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-MMP3 antibody
MMP3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MMP3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide derived from human MMP3 Peptide available as ab134501.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53015の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (3) |
1/50 - 1/100.
|
WB | (2) |
1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
|
ICC/IF | (1) |
Use a concentration of 5 µg/ml.
|
特記事項 |
---|
IHC-P
1/50 - 1/100. |
WB
1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
-
機能
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
参照データベース
- Entrez Gene: 4314 Human
- Omim: 185250 Human
- SwissProt: P08254 Human
- Unigene: 375129 Human
-
別名
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase 3 preproprotein antibody
see all
画像
-
All lanes : Anti-MMP3 antibody (ab53015) at 1/500 dilution
Lane 1 : Extracts from 293 cells, untreated.
Lane 2 : Extracts from 293 cells, treated with the immunising peptide.
Predicted band size: 54 kDa
Observed band size: 54 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma labeling MMP3 with ab53015 at 1/100 dilution. Tissue was fixed with formaldehyde; heat-mediated antigen retrieval was performed using citrate buffer (pH 6.0). The tissue was blocked with 1% serum for 1 hour at 25°C. A biotin conjugated polyclonal goat anti-rabbit secondary antibody was used at 1/200 dilution.
-
ICC/IF image of ab53015 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53015, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP3 antibody at a dilution of 1/50. Left: without immunizing peptide; Right: with immunizing peptide.
-
Top image: Isotype control Middle image: Unstimulated cells Bottom image: IL-1 beta stimulated cells (24 hours, 10 ng/ml)
ab53015 staining MMP3 in Human synovial fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in methanol for 15 minutes at -20°C prior to blocking in 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours at 25°C. The secondary antibody was Cy5®-conjugated Goat anti-Rabbit polyclonal, diluted 1/200.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (111)
ab53015 は 111 報の論文で使用されています。
- Du X et al. Circular RNA hsa_circ_0083756 promotes intervertebral disc degeneration by sponging miR-558 and regulating TREM1 expression. Cell Prolif 55:e13205 (2022). PubMed: 35187741
- Yeo C et al. Chaenomeles Fructus (CF), the Fruit of Chaenomeles sinensis Alleviates IL-1β Induced Cartilage Degradation in Rat Articular Chondrocytes. Int J Mol Sci 23:N/A (2022). PubMed: 35457176
- Chen GY et al. Network Pharmacology-Based Strategy to Investigate the Mechanisms of Cibotium barometz in Treating Osteoarthritis. Evid Based Complement Alternat Med 2022:1826299 (2022). PubMed: 35873632
- Sadowska M et al. Spreading Depressions and Periinfarct Spreading Depolarizations in the Context of Cortical Plasticity. Neuroscience 453:81-101 (2021). PubMed: 33227236
- Lin J et al. SIRT3 mitigates intervertebral disc degeneration by delaying oxidative stress-induced senescence of nucleus pulposus cells. J Cell Physiol 236:6441-6456 (2021). PubMed: 33565085