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    mmp14-antibody-ep1264y-ab51074.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-MMP14 抗体 [EP1264Y] (ab51074)

  • Datasheet
  • SDS
Reviews (19)Q&A (8)References (112)

Product price, shipping and contact information

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Immunoprecipitation - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Flow Cytometry - Anti-MMP14 antibody [EP1264Y] (ab51074)
  • Anti-MMP14 antibody [EP1264Y] (ab51074)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1264Y] to MMP14
  • Suitable for: Flow Cyt, IHC-P, IP, WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

こちらの製品もご検討ください

一次抗体
Product image
Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)
ELISA
Product image
Mouse Cathepsin B ELISA Kit (ab119585)
タンパク質
Recombinant human MMP14 protein (Active) (ab157068)

関連製品

製品の概要

  • 製品名

    Anti-MMP14 antibody [EP1264Y]
    MMP14 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EP1264Y] to MMP14
  • 由来種

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide within Human MMP14 aa 150-250. The exact sequence is proprietary.
    Database link: P50281
    (Peptide available as ab185123)

  • ポジティブ・コントロール

    • IHC: Human breast carcinoma, endometrium carcinoma and kidney tissues. WB: Human fetal spleen, esophagus and lung cancer tissue lysates, mouse and rat spleen tissue lysates; A431 and Caco-2 whole cell lysate. Flow Cyt: A549 cells. IP: A431 cell lysate.
  • 特記事項

    The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EP1264Y
  • アイソタイプ

    IgG
  • 研究分野

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Matrix Metalloproteinases
    • MMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • ECM enzymes
    • MMPs
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • MMPs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Alternative Versions

    • Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)
    • HRP Anti-MMP14 antibody [EP1264Y] (ab194242)
    • Anti-MMP14 antibody [EP1264Y] - BSA and Azide free (ab271840)
  • Assay kits

    • MMP14 Inhibitor Screening Assay Kit (Colorimetric) (ab139454)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Immunizing Peptide (Blocking)

    • MMP14 peptide (ab185123)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A-431 whole cell lysate (ab30132)
  • Recombinant Protein

    • Recombinant human MMP14 protein (Active) (ab157068)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Mouse
Rat
Human
All applications
Cow
アプリケーション Abreviews 特記事項
Flow Cyt (1)
1/20.
IHC-P (4)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/20.

For unpurified use at 1/100.

WB (7)
1/5000. Predicted molecular weight: 66 kDa.Can be blocked with MMP14 peptide (ab185123).

For unpurified use at 1/2000.

ICC/IF (6)
Use at an assay dependent concentration.
特記事項
Flow Cyt
1/20.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/20.

For unpurified use at 1/100.

WB
1/5000. Predicted molecular weight: 66 kDa.Can be blocked with MMP14 peptide (ab185123).

For unpurified use at 1/2000.

ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • 組織特異性

    Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • 配列類似性

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメイン

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾

    The precursor is cleaved by a furin endopeptidase.
  • 細胞内局在

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P50281 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 281915 Cow
    • Entrez Gene: 4323 Human
    • Entrez Gene: 17387 Mouse
    • Entrez Gene: 81707 Rat
    • Omim: 600754 Human
    • SwissProt: Q9GLE4 Cow
    • SwissProt: P50281 Human
    • SwissProt: P53690 Mouse
    • SwissProt: Q10739 Rat
    • Unigene: 2399 Human
    • Unigene: 280175 Mouse
    • Unigene: 10371 Rat
    see all
  • 別名

    • Matrix metallopeptidase 14 (membrane inserted) antibody
    • Matrix metalloproteinase 14 antibody
    • Matrix metalloproteinase-14 antibody
    • Membrane type 1 matrix metalloproteinase antibody
    • Membrane type 1 metalloprotease antibody
    • Membrane type matrix metalloproteinase 1 antibody
    • Membrane-type matrix metalloproteinase 1 antibody
    • Membrane-type-1 matrix metalloproteinase antibody
    • MMP 14 antibody
    • MMP X1 antibody
    • MMP-14 antibody
    • MMP-X1 antibody
    • Mmp14 antibody
    • MMP14_HUMAN antibody
    • MMPX1 antibody
    • MT MMP 1 antibody
    • MT-MMP 1 antibody
    • MT1 MMP antibody
    • MT1-MMP antibody
    • MT1MMP antibody
    • MTMMP 1 antibody
    • MTMMP1 antibody
    see all

画像

  • Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    All lanes : Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/2000 dilution

    Lane 1 : A431 wild-type cell lysate
    Lane 2 : A431 MMP14 knockout cell lysate
    Lane 3 : Caco-2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab51074 observed at 54 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab51074 was shown to react with MMP14 in A431 wild-type cells in Western blot. Loss of signal was observed when MMP14 knockout sample was used. A431 wild-type and MMP14 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% Milk in TBS-T (0.1% Tween®) before incubation with ab51074 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    All lanes : Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/2000 dilution (unpurified)

    Lane 1 : Human fetal spleen tissue lysate
    Lane 2 : Human lung cancer tissue lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 66 kDa
    Observed band size: 60,63 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    63 kDa: pro-form; 60 kDa: active form.

    MCF7 is a MMP14 negative or weakly expressed cell line (PMID: 25977338 and PMID: 19208838).

  • Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    All lanes : Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/5000 dilution (purified)

    Lane 1 : Human fetal spleen lysate
    Lane 2 : Human lung cancer lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : Human esophagus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium carcinoma tissue sections labeling MMP14 with purified ab51074 at 1/100 dilution (1.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counterstain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution.

    PBS instead of the primary antibody was used as the negative control (inset).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP14 antibody [EP1264Y] (ab51074)This image is courtesy of an anonymous Abreview

    ab51074 (unpurified) at 1/500 staining human kidney tissue sections by IHC-P.

    The tissue was formaldehyde fixed and a heat mediated antigen retrieval step (in Tris/EDTA) was performed. The tissue was then blocked with serum and incubated with the primary antibody. A biotinylated donkey anti-rabbit IgG was used as the secondary.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Immunocytochemistry/ Immunofluorescence - Anti-MMP14 antibody [EP1264Y] (ab51074)

    ab51074 staining MMP14 in HT-1080 (human fibrosarcoma epithelial cell) cells by ICC/IF (Immunocytochemistry/Immunofluorescence).

    Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 1/1000 dilution (0.2 μg/ml). An Alexa Fluor® 488 Goat anti-rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, ab195889) was used as the counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic and weakly membranous staining in HT-1080 cell line.

    Negative control (bottom panels): MCF7 PMID: 19208838.

  • Immunoprecipitation - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Immunoprecipitation - Anti-MMP14 antibody [EP1264Y] (ab51074)

    ab51074 (purified) at 1/20 dilution (2 µg) immunoprecipitating MMP14 in A431 (human epidermoid carcinoma) whole cell lysate.

    Lane 1: A431 whole cell lysate 10ug
    Lane 2: ab51074 + A431 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab51074 in A431 whole cell lysate

    For western blotting, ab131366 VeriBlot for IP (HRP) was used for detection (1/1000).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Flow Cytometry - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Flow Cytometry - Anti-MMP14 antibody [EP1264Y] (ab51074)

    Flow cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) / HT-1080 (Human fibrosarcoma epithelial cell, Right) cells labeling MMP14 with ab51074 at 1/200 dilution (0.1 μg) (red). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Rabbit monoclonal IgG (ab172730) / black was used as the isotype control. Cells incubated with secondary antibody only (blue) was used as the unlabeled control. Gated on viable cells.

    Positive control (Right panel): HT-1080 cells.

    Negative control (Left panel): MCF7 cells.

  • Anti-MMP14 antibody [EP1264Y] (ab51074)
    Anti-MMP14 antibody [EP1264Y] (ab51074)

プロトコール

  • IHC-P protocol

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (112)

    ab51074 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab51074 は 112 報の論文で使用されています。

    • Klepfish M  et al. LOXL2 Inhibition Paves the Way for Macrophage-Mediated Collagen Degradation in Liver Fibrosis. Front Immunol 11:480 (2020). PubMed: 32296422
    • Thuault S  et al. A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells. Sci Rep 10:6787 (2020). PubMed: 32321993
    • Gissi C  et al. Extracellular vesicles from rat-bone-marrow mesenchymal stromal/stem cells improve tendon repair in rat Achilles tendon injury model in dose-dependent manner: A pilot study. PLoS One 15:e0229914 (2020). PubMed: 32163452
    • Yao J  et al. Single-cell transcriptomic analysis in a mouse model deciphers cell transition states in the multistep development of esophageal cancer. Nat Commun 11:3715 (2020). PubMed: 32709844
    • Kasurinen A  et al. High tissue MMP14 expression predicts worse survival in gastric cancer, particularly with a low PROX1. Cancer Med 8:6995-7005 (2019). PubMed: 31560170
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 27 Abreviews or Q&A

    Question

    This customer raises an enquiry about ab51074 (Anti-MMP14 antibody [EP1264Y]), he wants to know the more information about this immunogen, could you please offer any information to him (ex. N-terminal or C-terminal), thanks for your kindly help.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 07 2012

    Answer

    Thank you for contacting us.


    According to our internal records, this immunogen isnot from the N-terminus. In addition, itwill recognise pro and mature forms.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 07 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MMP14 antibody [EP1264Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - NP40
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 11 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MMP14 antibody [EP1264Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse vascular smooth muscle cells)
    Permeabilization
    Yes - NP40
    Specification
    Mouse vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 11 2021

    Western blot abreview for Anti-MMP14 antibody [EP1264Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hiPSC-Brain Microvascular Endothelial Cells)
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Loading amount
    20 µg
    Specification
    hiPSC-Brain Microvascular Endothelial Cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Mrs. Alessandra Granata

    Verified customer

    投稿 Dec 30 2019

    Western blot abreview for Anti-MMP14 antibody [EP1264Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Melanoma)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    15 µg
    Specification
    Melanoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 19 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MMP14 antibody [EP1264Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (vertebrae)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: 10 mg/mL hyaluronidase+100mU/mL chondrotinase ABC
    Permeabilization
    No
    Specification
    vertebrae
    Blocking step
    (agent) for 10 minute(s) · Concentration: 100% · Temperature: 21°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 14 2019

    Western blot abreview for Anti-MMP14 antibody [EP1264Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (ARPE-19)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris Gel)
    Loading amount
    10 µg
    Treatment
    125 ug/ml cigarette smoke extract for 24hrs
    Specification
    ARPE-19
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 13 2019

    Western blot abreview for Anti-MMP14 antibody [EP1264Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 10 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MMP14 antibody [EP1264Y]

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brain)
    Permeabilization
    Yes - 0.1% Triton-X in PBS
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Daniel Lustberg

    Verified customer

    投稿 May 25 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MMP14 antibody [EP1264Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Bos taurus Tissue sections (Airway)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
    Permeabilization
    No
    Specification
    Airway
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 23 2015

    1-10 of 27 Abreviews or Q&A

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