The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 - 5 µg/ml. Predicted molecular weight: 54 kDa.
Use a concentration of 10 - 20 µg/ml.
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
Seems to be specific to breast carcinomas.
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age. Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Secreted > extracellular space > extracellular matrix.
Anti-MMP13 antibody (ab75606) at 5 µg/ml + Conditioned, serum-free medium from (TPA-treated) rat CCL-4 cells
Predicted band size: 54 kDa Observed band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody (ab75606)This image is courtesy of an anonymous Abreview
ab75606 staining MMP13 in Rat shoulder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked witha vector kit for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in antibody diluent) for 16 hours at 4°C. A undiluted Biotin-conjugated polyclonal was used as the secondary antibody.
McDonald H et al. Hexarelin treatment preserves myocardial function and reduces cardiac fibrosis in a mouse model of acute myocardial infarction. Physiol Rep6:e13699 (2018).
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