Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Goat Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Permeabilization
Yes - 3% Hydrogen Peroxide
Specification
Goat Lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: VECTASTAIN ABC Reagent
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Chenghao Zhang
Verified customer
投稿 Jan 31 2019