製品の概要

  • 製品名

  • 製品の詳細

    Goat polyclonal to MKP-1
  • 由来種

    Goat
  • 特異性

    The immunogen sequence for ab1351, anti MKP-1 antibody (aka DUSP1), shares 100% sequence homology to MKP-2 (aka DUSP4).

  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human MKP-1 aa 355-367 (C terminal). The immunogen sequence for ab1351, anti MKP-1 antibody (aka DUSP1), shares 100% sequence homology to MKP-2 (aka DUSP4).
    Sequence:

    SYLQSPITTSPSC


    Database link: P28562

  • ポジティブ・コントロール

    • Recombinant Human MKP-1 protein (ab114331) can be used as a positive control in WB. WB: HeLa cell lysate

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml.

ターゲット情報

  • 機能

    Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
  • 組織特異性

    Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
  • 配列類似性

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Information by UniProt
  • 参照データベース

  • 別名

    • CL 100 antibody
    • CL100 antibody
    • Dual Specificity Phosphatase 1 antibody
    • Dual specificity protein phosphatase 1 antibody
    • Dual specificity protein phosphatase hVH1 antibody
    • DUS1_HUMAN antibody
    • DUSP 1 antibody
    • Dusp1 antibody
    • HVH1 antibody
    • MAP kinase phosphatase 1 antibody
    • Mitogen-activated protein kinase phosphatase 1 antibody
    • MKP-1 antibody
    • MKP1 antibody
    • Protein tyrosine phosphatase CL100 antibody
    • Protein-tyrosine phosphatase CL100 antibody
    • PTPN10 antibody
    • Serine/threonine specific protein phosphatase antibody
    • VH1 antibody
    see all

画像

  • Anti-MKP-1 antibody (ab1351) at 1 µg/ml + HeLa cell lysate at 35 µg

参考文献

This product has been referenced in:

  • Unni AM  et al. Hyperactivation of ERK by multiple mechanisms is toxic to RTK-RAS mutation-driven lung adenocarcinoma cells. Elife 7:N/A (2018). Read more (PubMed: 30475204) »
  • Shen J  et al. DUSP1 inhibits cell proliferation, metastasis and invasion and angiogenesis in gallbladder cancer. Oncotarget 8:12133-12144 (2017). Read more (PubMed: 28129656) »
See all 5 Publications for this product

レビューと Q&A

1-10 of 11 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Chicken Cell lysate - whole cell (Thrombocyte)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
2 µg
Specification
Thrombocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Mrs. Katie Elliott

Verified customer

投稿 Sep 29 2016

Application
Immunocytochemistry
Sample
Human Cultured Cells (Glioblastoma cells (U87MG, LN18,..) (all human))
Permeabilization
Yes - Triton x
Specification
Glioblastoma cells (U87MG, LN18,..) (all human)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Mr. Matthias Dedobbeleer

Verified customer

投稿 May 04 2016

Question
Answer

We recommend using a permeablization step with this product in IHC-P. Permeabilize with 0.25% Triton X-100 for 10 minutes at room temperature. Perform this step after antigen retrieval and before blocking.

Read More
Application
Western blot
Sample
Rat Tissue lysate - whole (spinal cord homogenate)
Gel Running Conditions
Reduced Denaturing (10% SDS_PAGE)
Loading amount
20 µg
Treatment
sham or nerve transected
Specification
spinal cord homogenate
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Jun 17 2008

Application
Western blot
Sample
Rat Cell lysate - whole cell (HAPI cell line)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
400000 cells
Treatment
LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Specification
HAPI cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Jun 13 2008

Application
Western blot
Sample
Mouse Cell lysate - whole cell (BV2 cell line)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
400000 cells
Treatment
LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Specification
BV2 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Jun 10 2008

Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Microglia)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
400000 cells
Treatment
LPS 50 ng/mL or LPS 50 ng/mL +UO 10 uM
Specification
Primary Microglia
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Jun 10 2008

Answer

Thank you for your enquiry. I apologize for the delay in answering your question due to the holidays. The protocol used to test this antibody is below. 35ug protein per lane was loaded and its concentration would have been 2.5mg/ml in SDS Page loading buffer. The cells were not stimulated. - Lysis. Cell pellets were washed with ice-cold PBS. 1 ml of RIPA buffer was added per 1E8 cells and incubated on ice for 20 min, vortexing 2-3 times, briefly. The lysate was aliquotted into 1.5 ml microfuge tubes and centrifuged at 13,000 rpm for 5 min in a microfuge. The supernatant was transferred into clean tubes and its protein concentration was measured with BioRad protein assay. The concentration was then adjusted to 5 mg/ml with RIPA lysis buffer. An equal volume of 2 x SDS sample buffer was then added and the cell lysate was boiled for 5 minutes. Lysates were stored at -80C until use.(RIPA buffer = 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 µg/ml Aprotinin, 5 µg/ml Leupeptin, 1% Triton X-100, 1% Sodium deoxycholate, 0.1% SDS). - SDS PAGE. Samples were run at 200V constant on a 12% acrylamide SDS-PAGE mini gel - using Biorad Mini-Protean 3 kit and protocols. Before loading samples had 5% (v/v) 2-ME added and were boiled for 3 minutes. - Transfer. We used a Biorad Mini Trans-Blot, constant 100 V for 1 hour. Transfer Buffer was 20 mM Tris pH 8.0, 150 mM Glycine, 10% Methanol. We transferred to Millipore PVDF membrane and stained with Ponceau Red to evaluate the transfer. - Staining. The membrane was blocked in 2.5% skimmed milk in TBS-T (TBS + 0.05% Tween-20) for 1 hr at room temperature with agitation. Primary antibody was incubated for 1 hr at room temperature with agitation. We used anti-goat-HRP for 1 hr at room temperature with agitation. We washed with TBST three times after primary and secondary antibody, each wash lasting for 5-10 mins. ECL-plus (Amersham) was used rather than ECL, which is considerably more sensitive. Final detection was on autoradiography film.

Read More

Answer

Thank you for your enquiry. The Western on the datasheet is detecting endogenous DUSP1 in Hela. In order to further troubleshoot why this antibody is not giving you any signal, it would be helpful if you could fill out the form at the link I am including below and submit the form. This will give us further details regarding the protocol you are using. The form can be found at: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=1351&mode=questionaire Please do not hesitate to contact us if you need anything further.

Read More

Answer

Thank you for your enquiry and for your patience. The originator of this antibody has informed me that they only observed a single band in HeLa lysate and we have not had any other feedback yet regarding the use of ab1351. However, looking at the sequences it is possible that ab1351 could recognize DUSP4. This has not been tested. If you have any more questions, please do contact us again.

Read More

1-10 of 11 Abreviews or Q&A

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