Anti-MKK7 (phospho S271 + T275) 抗体 (ab4762)
Key features and details
- Rabbit polyclonal to MKK7 (phospho S271 + T275)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MKK7 (phospho S271 + T275) antibody
MKK7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MKK7 (phospho S271 + T275) -
由来種
Rabbit -
特異性
This antibody does not react with other MEK isoforms. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human MKK7.
(Peptide available asab5742) -
ポジティブ･コントロール
- Fusion protein expressing MEK 7.
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特記事項
This product was previously labelled as MEK7
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit serum by sequential epitope specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 7. The final product is generated by affinity chromatography using an MEK 7 derived peptide that is phosphorylated at serine 271 and threonine 275. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab4762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 38 kDa. |
ターゲット情報
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機能
Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. -
組織特異性
Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Activated by phosphorylation on Ser/Thr. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5609 Human
- Entrez Gene: 26400 Mouse
- Omim: 603014 Human
- SwissProt: O14733 Human
- SwissProt: Q8CE90 Mouse
- Unigene: 531754 Human
- Unigene: 3906 Mouse
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別名
- c-Jun N-terminal kinase kinase 2 antibody
- Dual specificity mitogen activated protein kinase kinase 7 antibody
- Dual specificity mitogen-activated protein kinase kinase 7 antibody
see all
画像
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Western blot - Anti-MKK7 (phospho S271 + T275) antibody (ab4762)Peptide Competition: Extracts prepared from background extracts with tagged fusion protein expressing MEK 7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50
µ
データシートおよび資料
参考文献 (2)
ab4762 は 2 報の論文で使用されています。
- Xie SJ et al. Inhibition of the JNK/MAPK signaling pathway by myogenesis-associated miRNAs is required for skeletal muscle development. Cell Death Differ 25:1581-1597 (2018). PubMed: 29449644
- Sheng X et al. Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK. PLoS Pathog 13:e1006534 (2017). PubMed: 28753655
