Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 2.5% milk in PBS-Tween20
Secondary antibody
Name
Dilution
1/1000
Detection
Detection method
fluorescence scanning
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 180 kDa Non-specific: 150, 100, 70, 40, 30 kDa
Additional data
Additional Notes
The secondary antibody (Alexa647-conjugated) was detected using a fluorescence scanner equipped with a red laser (633 nm), at a PMT of 700V.
This antibody detects a number of bands of various molecular mass, suggesting that it may bind to several antigens and is not specific.
This antibody detects a number of bands of various molecular mass, suggesting that it may bind to several antigens and is not specific.
Abcam response
We apreciate the valuable data provided in this Abreview. As stated on the datasheed we would not expect this antibody to be suitable for WB application. Furthermore Apteronotus leptorhynchus is an untested species. We would be pleased to recive feedback for this sample type in a tested application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 06 2012