Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Mitotic Cells 抗体 [8B3G] (ab8956)

Reviews (2)Q&A (4)References (2)
Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (ab8956)
  • Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)
  • Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)

Key features and details

  • Mouse monoclonal [8B3G] to Mitotic Cells
  • Suitable for: Flow Cyt, IHC-Fr
  • Reacts with: Human, Zebrafish
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-Mitotic Cells antibody [8B3G]

  • 製品の詳細

    Mouse monoclonal [8B3G] to Mitotic Cells

  • 由来種

    Mouse

  • 特異性

    Strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) this antibody clearly distinguishes these M-phase cells from cell at other stages of the cell cycle. Dynamic information can be obtained by combining BrdU incorporation with antibody staining, which can distinguish and quantitate the four major fractions of the cell cycle. This antibody can be used for flow cytometric analyses and immunocytochemistry, it is not suitable for immunoblotting.

  • アプリケーション

    適用あり: Flow Cyt, IHC-Frmore details

  • 種交差性

    交差種: Human, Zebrafish

  • 免疫原

    Tissue, cells or virus corresponding to Human Mitotic Cells. Total cell lysate of the human bladder carcinoma cell line T24.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8956の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
IHC-Fr
Use at an assay dependent concentration.
特記事項
Flow Cyt
Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
IHC-Fr
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    The life cycle of a eukaryotic cell consists of various phases, two of which can easily be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques. In between the M and S phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a ‘quiescent’ phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S and from G2 to M-phase.

画像

  • Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (ab8956)
    Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (ab8956)
    Dual parameter flow cytometric analysis of human colon cancer HT29 cells stained with monoclonal antibody 8B3G and propidium iodide (PI). The mitotic cell fraction is encircled.

     

  • Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)
    Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)
    ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone:Methanol 1:1. ab8956 used at 1:50 dilution and incubated for 45 minutes at room temperature.
  • Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)
    Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)
    ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone:Methanol 1:1. ab8956 used at 1:50 dilution and incubated for 45 minutes at room temperature.

参考文献 (2)

ab8956 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab8956 は 2 報の論文で使用されています。

  • Wiesmann N  et al. Phosphoproteome Profiling Reveals Multifunctional Protein NPM1 as part of the Irradiation Response of Tumor Cells. Transl Oncol 12:308-319 (2019). PubMed: 30453269
  • Delobel P  et al. Cell-cycle markers in a transgenic mouse model of human tauopathy: increased levels of cyclin-dependent kinase inhibitors p21Cip1 and p27Kip1. Am J Pathol 168:878-87 (2006). Mouse . PubMed: 16507903

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Mitotic Cells antibody [8B3G]

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.3% TritonX-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Read More

Abcam user community

Verified customer

投稿 Feb 05 2013

Western blot abreview for Anti-Mitotic Cells antibody [8B3G]

 Below Average
Abreviews
abreview image
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Dec 06 2012

Question

Thank you very much for the detailed information. I admit that we did
not have too high expectations regarding this antibody in WB, however
because the results obtained in IHC-Fr were inconclusive (unspecific,
binding in many areas), I tried to see which kind, and how many
different antigens does this antibody recognize. As it looks like, it
recognized several bands, possibly signifying several very different
proteins - which certainly would explain the unspecific binding observed
in IHC.
Regarding the secondary antibody: indeed a different antibody was used:
goat polyclonal to mouse IgM - DyLight 488 (ab97007) Thank you for
catching this error in the Abreview! Below I have revised the Abreview
to reflect the correct sequence of antibodies:

Read More
Answer

Thank you very much for your reply and providing this additional information. We appreciate the time that was spent providing this Abreview.
The Abreview has now been published and you have received the reward points.
Please do not hesitate to contact us with further questions.

Read More

Question

The immunogen is stated as being "Total cell lysate of the human bladder carcinoma cell line T24." Are there more specifics regarding this? What specifically is the antibody binding to? Are there any references regarding this clone?

Read More
Answer

The mice that were used to produce ab8956 were indeed immunized with a total cell lysate of proliferating T24. Since the antibody behaves rather poorly in immunoblotting or the amount of its antigen in the cell is low, we have not been able to clearly identify the protein to which ab8956 binds. We have performed flow cytometric analyses with ab8956 that revealed a particularly strong reactivity in mitotic cells (see datasheet}. Unfortunately ab8956 has not yet been published with.

Read More

Question

Regarding this product, could you please tell me the answer to: What does this antibody detect in the cell? Why is it mitosis specific?

Read More
Answer

Thank you for your patience in awaiting a response. Unfortunately this antibody acts poorly in immunoblotting studies. We therefore have not been able to identify the antigen that the antibody recognizes. Immunofluorescence studies and flow cytometric analyses have show the highest expression of the antigen in mitotic cells. If you have any further questions then please get back in touch with us.

Read More

Question

What does this antibody detect in the cell? Why is it mitosis-specific? What is the recommended concentration for staining for FACS? Thank you.

Read More
Answer

Thank you for your enquiry. Although suitable for FACS, due to the amount supplied I have removed this as a tested application from the on line data sheet. If you have any further questions then please do not hesitate to get back in touch with us. I apologise for any inconvenience that this has caused.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com