Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7960] to Mitofusin 1 - BSA and Azide free
- Suitable for: Flow Cyt, IP, WB
- Reacts with: Human
製品名Anti-Mitofusin 1 antibody [EPR7960] - BSA and Azide free
Mitofusin 1 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7960] to Mitofusin 1 - BSA and Azide free
アプリケーション適用あり: Flow Cyt, IP, WBmore details
適用なし: ICC/IF or IHC-P
Synthetic peptide within Human Mitofusin 1 aa 550-650. The exact sequence is proprietary.
ab248315 is the carrier-free version of ab129154 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248315 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 84 kDa).|
機能Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
組織特異性Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
配列類似性Belongs to the mitofusin family.
翻訳後修飾Ubiquitinated by MARCH5.
細胞内局在Cytoplasm and Mitochondrion outer membrane.
- Information by UniProt
- Fzo homolog antibody
- Hfzo1 antibody
- Hfzo2 antibody
All lanes : Anti-Mitofusin 1 antibody [EPR7960] (ab129154) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 84 kDa
This data was developed using ab129154, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.