製品の概要

  • 製品名
    Mitochondrial Staining Kit - Orange Fluorescence - Cytopainter
    Mitochondria キット 製品一覧
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプ
    Cell-based
  • 製品の概要

    Mitochondrial Staining Kit - Orange Fluorescence | Cytopainter (ab112144) is designed to label mitochondria in live cells with orange fluorescence at Ex/Em = 545/575nm (TRITC filter-compatible). This kits uses a proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency. ab112144 can be readily adapted for many different types of fluorescence platforms, such as microplate assays, immunocytochemistry and flow cytometry.


    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.


    Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide

  • 特記事項

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signalling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • 試験プラットフォーム
    Fluorescence microscope

製品の特性

画像

  • CytoPainter Mitochondrial Staining Kit - Orange Fluorescence (ab112144). Image of U2OS cells stained with ab112144 in a black 96-well plate

プロトコール

参考文献

ab112144 has not yet been referenced specifically in any publications.

レビューと Q&A

1-3 of 3 Abreviews or Q&A

Abreviews
We have used this kit for staining mitochondria in living ES cells and found that it woks well.
The product is just easy to use. Note that once you permeabilize the cells before fixation, you will lose signals.

Abcam user community

Verified customer

投稿 Mar 12 2018

Answer

J'ai reçu confirmation que les kits de marquage fluorescent des mitochondries CytoPainter :
1) peuvent être utilisés sur des cellules vivantes,
2) ne stoppent pas la division cellulaire,
3) Ne sont à priori pas toxiques,
4) Peuvent être utilisés avec d'autres marqueurs fluorescents.

Les lien vers les fiches techniques des kits "CytoPainter Mitochondrial Staining" sont:
https://www.abcam.com/ab112143 marquage vert (490/520 nm),
https://www.abcam.com/ab112144 marquage orange (545/575 nm),
https://www.abcam.com/ab112145 marquage rouge (580/600 nm)

Le lien vers la page générale des marqueurs CytoPainter est : https://www.abcam.com/cytopainter.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Answer

Merci de nous avoir contactés.

Le Draq5 se lie à l'ADN et empêche donc malheureusement la division cellulaire.

Un autre produit me semble intéressant: CytoPainter Mitochondrial Staining Kit - Orange Fluorescence (ab112144), www.abcam.com/ab112144.

Je me renseigne auprès du laboratoire si il conviendrait à vos travaux et je vous contacterai de nouveau quand j'aurai reçu une réponse.

Merci pour votre patience.

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