Mitochondria Isolation Kit for Cultured Cells (ab110170)
Key features and details
- Assay time: 1 hr
製品の概要
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製品名
Mitochondria Isolation Kit for Cultured Cells
Mitochondria Isolation キット 製品一覧 -
全工程の試験時間
1h 00m -
種交差性
交差種: Mammals -
製品の概要
ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Available with a dounce homogenizer as kit ab110171.
Mitochondrial isolation protocol summary:
- suspend cells in reagent A and incubate for 10 min
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant, repeat
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in reagent C and analyze or freeze
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 kit Reagent A 1 x 50ml Reagent B 1 x 50ml Reagent C 1 x 10ml -
研究分野
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別名
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
関連製品
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Related Products
画像
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Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110170 (MS852) Mitochondria Isolation Kit for Cultured Cells were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (67)
ab110170 は 67 報の論文で使用されています。
- Suárez-Rivero JM et al. Pterostilbene in Combination With Mitochondrial Cofactors Improve Mitochondrial Function in Cellular Models of Mitochondrial Diseases. Front Pharmacol 13:862085 (2022). PubMed: 35370630
- Morioka E et al. Mitochondrial LETM1 drives ionic and molecular clock rhythms in circadian pacemaker neurons. Cell Rep 39:110787 (2022). PubMed: 35545046
- Toda T et al. Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria. Commun Biol 5:453 (2022). PubMed: 35552531
- Sohn YS et al. A peptide-derived strategy for specifically targeting the mitochondria and ER of cancer cells: a new approach in fighting cancer. Chem Sci 13:6929-6941 (2022). PubMed: 35774163
- Dong L et al. Mannose ameliorates experimental colitis by protecting intestinal barrier integrity. Nat Commun 13:4804 (2022). PubMed: 35974017