Anti-Mitochondria 抗体 [AE-1] (ab215892)
Key features and details
- Mouse monoclonal [AE-1] to Mitochondria
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Mitochondria antibody [AE-1]
Mitochondria 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AE-1] to Mitochondria -
由来種
Mouse -
アプリケーション
適用あり: ICC, WB, IHC-Pmore details -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Tissue, cells or virus corresponding to Human Mitochondria. Semi-purified mitochondrial preparation
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ポジティブ・コントロール
- IHC: Human tonsil and pancreas tissues. ICC: Human HeLa cells WB: Human HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
ab215892 is purified from Bioreactor Concentrate by Protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
AE-1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab215892の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 0.5 - 1 µg/ml.
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WB |
Use a concentration of 0.25 - 0.5 µg/ml.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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ICC
Use a concentration of 0.5 - 1 µg/ml. |
WB
Use a concentration of 0.25 - 0.5 µg/ml. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Mitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales". -
細胞内局在
Mitochondrial
画像
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Anti-Mitochondria antibody [AE-1] (ab215892) at 0.5 µg/ml + HeLa cells (human epithelial cell line from cervix adenocarcinoma)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling mitochondria with ab215892 at 2µg/ml dilution. Heat mediated antigen retrieval performed with tris/EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling mitochondria with ab215892 at 2µg/ml dilution. Heat mediated antigen retrieval performed with tris/EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunocytochemistry confocal immunoflourescence of HeLa cells (human epithelial cell line from cervix adenocarcinoma) stained with ab215892 2µg/ml in green, and nuclear counter staining in RedDot red.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling Mitochondria with ab215892 at 1µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Mitochondria with ab215892 at 1µg/ml.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab215892 は論文での使用が確認できていません。