Anti-Mitochondria 抗体 [113-1] - BSA and Azide free (ab92824)
Key features and details
- Mouse monoclonal [113-1] to Mitochondria - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Mitochondria antibody [113-1] - BSA and Azide free
Mitochondria 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [113-1] to Mitochondria - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Human cell homogenate
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ポジティブ・コントロール
- WB: HeLa cell lysate. ICC/IF: HeLa and U87 cells. IHC-P: Human breast cancer tissue. Flow Cyt: HepG2 cells.
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特記事項
This antibody is an excellent marker for Human cells in xenographic model research.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
一次抗体 備考
This antibody is an excellent marker for Human cells in xenographic model research. -
ポリ/モノ
モノクローナル -
クローン名
113-1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92824の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 60 kDa.
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IHC-P | (4) |
1/1000.
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ICC/IF | (3) |
1/800.
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Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000. Detects a band of approximately 60 kDa. |
IHC-P
1/1000. |
ICC/IF
1/800. |
Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
Mitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales". -
細胞内局在
Mitochondrial
画像
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Immunostained human cells in spleen and temporal artery (TA) xenograft sections demonstrate persistent human PBMC colonization at 28 days
Mitochondria in spleen (B) and temporal artery xenografts (E) were detected using ab92824 at 1/400 dilution in immunohistochemical analysis.
Magnification: 400X for spleen, 200X for TA grafts and 800X for insets.
(From Figure 2 of Chen et al)
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Anti-Mitochondria antibody [113-1] - BSA and Azide free (ab92824) at 1/1000 dilution + HeLa lysate
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ICC/IF image of ab92824 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92824, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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IHC image of ab92824 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92824, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Overlay histogram showing HepG2 cells stained with ab92824 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92824, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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ab92824 staining mitochondria in the Human U87 glioblastoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 10% NBF, permeabilized with Triton X-100 and blocked with 2% serum for 90 minutes at 22°C. Samples were incubated with primary antibody (1/1000 in 0.2% BSA + 2% NGS) for 15 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG1 polyclonal was used as the secondary antibody (1/500). Co stained with ActinGreen, 2 drops/ml PBS, 10 min and Hoechst 2ug/ml in H2O 10 min
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (77)
ab92824 は 77 報の論文で使用されています。
- Alexander JF et al. Targeting the Meningeal Compartment to Resolve Chemobrain and Neuropathy via Nasal Delivery of Functionalized Mitochondria. Adv Healthc Mater 11:e2102153 (2022). PubMed: 35007407
- Chen K et al. Human Decidual Mesenchymal Stem Cells Obtained From Early Pregnancy Improve Cardiac Revascularization Postinfarction by Activating Ornithine Metabolism. Front Cardiovasc Med 9:837780 (2022). PubMed: 35242829
- Chen G et al. Apelin-13 Pretreatment Promotes the Cardioprotective Effect of Mesenchymal Stem Cells against Myocardial Infarction by Improving Their Survival. Stem Cells Int 2022:3742678 (2022). PubMed: 35355588
- Tontsch-Grunt U et al. Therapeutic impact of BET inhibitor BI 894999 treatment: backtranslation from the clinic. Br J Cancer 127:577-586 (2022). PubMed: 35444289
- Peng SY et al. Aldosterone Suppresses Endothelial Mitochondria through Mineralocorticoid Receptor/Mitochondrial Reactive Oxygen Species Pathway. Biomedicines 10:N/A (2022). PubMed: 35625856