製品の概要

  • 製品名

    MiTMAB™, dynamin I and dynamin II inhibitor
  • 製品の詳細

    Cell-permeable dynamin I and dynamin II inhibitor
  • 生理活性の詳細

    Cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 3.1 and 8.4 µM for inhibition of dynamin I and dynamin II GTPase, respectively). Targets the pleckstrin homology (PH) (lipid binding) domain. Competitive with phospholipid and non-competitive with GTP. Inhibits receptor-mediated and synaptic vesicle endocytosis (IC50 values are 19.9 and 2.2 µM, respectively). Inhibits cancer cell growth.
  • 特記事項

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. MiTMAB™ and OcTMAB™ are trademarks of Children's Medical Research Institute and Newcastle Innovation Ltd.
  • CAS 番号

    1119-97-7
  • 構造式

    Chemical Structure

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab120466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • ab66705 staining PAI1 in HeLa cells treated with MiTMAB™ (ab120466), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of MiTMAB™, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120466 (MiTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

参考文献

This product has been referenced in:

  • Harper CB  et al. Targeting membrane trafficking in infection prophylaxis: dynamin inhibitors. Trends Cell Biol 23:90-101 (2013). Read more (PubMed: 23164733) »
  • Joshi S  et al. The dynamin inhibitors MiTMAB and OcTMAB induce cytokinesis failure and inhibit cell proliferation in human cancer cells. Mol Cancer Ther 9:1995-2006 (2010). Read more (PubMed: 20571068) »
  • Quan A  et al. Myristyl trimethyl ammonium bromide and octadecyl trimethyl ammonium bromide are surface-active small molecule dynamin inhibitors that block endocytosis mediated by dynamin I or dynamin II. Mol Pharmacol 72:1425-39 (2007). Read more (PubMed: 17702890) »
  • Hill TA  et al. Long chain amines and long chain ammonium salts as novel inhibitors of dynamin GTPase activity. Bioorg Med Chem Lett 14:3275-8 (2004). Read more (PubMed: 15149689) »
See all 3 Publications for this product

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