Anti-MiTF (phospho S73) 抗体 (ab59201)
Key features and details
- Rabbit polyclonal to MiTF (phospho S73)
- Suitable for: WB
- Reacts with: African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-MiTF (phospho S73) antibody
MiTF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MiTF (phospho S73) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: African green monkey -
免疫原
Synthetic peptide corresponding to Human MiTF aa 150-250 (phospho S73).
Database link: O75030 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Store at -20°C. Stable for 12 months at -20°C -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59201の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
ターゲット情報
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機能
Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. -
組織特異性
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. -
関連疾患
Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete. -
配列類似性
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. -
細胞内局在
Nucleus. - Information by UniProt
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別名
- BHLHE32 antibody
- bHLHe32 antibody
- Class E basic helix-loop-helix protein 32 antibody
see all
画像
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All lanes : Anti-MiTF (phospho S73) antibody (ab59201) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract with phosphopeptide
Predicted band size: 59 kDa
Observed band size: 59 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab59201 は 4 報の論文で使用されています。
- Lan Y et al. Opsin 5 is a key regulator of ultraviolet radiation-induced melanogenesis in human epidermal melanocytes. Br J Dermatol 185:391-404 (2021). PubMed: 33400324
- Liu X et al. a-MSH-PE38KDEL Kills Melanoma Cells via Modulating Erk1/2/MITF/TYR Signaling in an MC1R-Dependent Manner. Onco Targets Ther 13:12457-12469 (2020). PubMed: 33299329
- Lee B et al. Swertiajaponin inhibits skin pigmentation by dual mechanisms to suppress tyrosinase. Oncotarget 8:95530-95541 (2017). PubMed: 29221146
- Han N et al. Evaluation of Fibroblast Growth Factor Receptor 2 Expression, Heterogeneity and Clinical Significance in Gastric Cancer. Pathobiology 82:269-79 (2015). PubMed: 26516773