Anti-MiTF 抗体 [D5] (ab3201)
Key features and details
- Mouse monoclonal [D5] to MiTF
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-MiTF antibody [D5]
MiTF 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [D5] to MiTF -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WB, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Dog非交差種: Rat
-
免疫原
corresponding to MiTF.
-
エピトープ
N-terminal -
ポジティブ・コントロール
- Flow Cyt: Malme-3 cells. ICC/IF: Malme-3 cells. WB: HeLa whole cell and nuclear lysate.
-
特記事項
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
-
精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
D5 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3201の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (1) |
Use at an assay dependent concentration.
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 59 kDa.
|
|
Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
特記事項 |
---|
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 59 kDa. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
ターゲット情報
-
機能
Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. -
組織特異性
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. -
関連疾患
Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete. -
配列類似性
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 4286 Human
- Entrez Gene: 17342 Mouse
- Omim: 156845 Human
- SwissProt: O75030 Human
- SwissProt: Q08874 Mouse
- Unigene: 166017 Human
- Unigene: 618266 Human
- Unigene: 333284 Mouse
see all -
別名
- BHLHE32 antibody
- bHLHe32 antibody
- Class E basic helix-loop-helix protein 32 antibody
see all
画像
-
All lanes : Anti-MiTF antibody [D5] (ab3201) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
Lane 2 : HeLa nuclear lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [D5] (ab3201)This image is courtesy of an anonymous Abreview
ab3201 staining MiTF in the SK-MEL-28 (Human cutaneous melanoma cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde and permeabilized with Triton X-100 0.5% in PBS. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/1000) was used as the secondary antibody. SK-MEL-28 cells was grown on chamber slide. MiTF was found to strictly localised in the nucleus. Cells were counter-stained with DAPI.
-
ICC/IF image of ab3201 stained Malme-3M (Human melanoma cell line) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab3201 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
-
Overlay histogram showing Malme-3 (Human skin fibroblast cell line) cells stained with ab3201 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3201, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (16)
ab3201 は 16 報の論文で使用されています。
- Swiatlowska P et al. Microtubules regulate cardiomyocyte transversal Young's modulus. Proc Natl Acad Sci U S A 117:2764-2766 (2020). PubMed: 31988123
- Li H et al. Thymocid®, a Standardized Black Cumin (Nigella sativa) Seed Extract, Modulates Collagen Cross-Linking, Collagenase and Elastase Activities, and Melanogenesis in Murine B16F10 Melanoma Cells. Nutrients 12:N/A (2020). PubMed: 32707654
- Jiang TX et al. Comparative regenerative biology of spiny (Acomys cahirinus) and laboratory (Mus musculus) mouse skin. Exp Dermatol 28:442-449 (2019). PubMed: 30734959
- Gong Y et al. SCF/SCFR signaling plays an important role in the early morphogenesis and neurogenesis of human embryonic neural retina. Development 146:N/A (2019). PubMed: 31548215
- Bai X et al. Generation of an integration-free induced pluripotent stem cell line (FDEENTi003-A) from a patient with pathological myopia. Stem Cell Res 39:101495 (2019). PubMed: 31376721