Anti-MiTF 抗体 [C5] (ab12039)
Key features and details
- Mouse monoclonal [C5] to MiTF
- Suitable for: ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-MiTF antibody [C5]
MiTF 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [C5] to MiTF -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WB, Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken -
免疫原
Recombinant fragment corresponding to Human MiTF (N terminal).
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ポジティブ・コントロール
- WB: Malme-3M whole cell lysate. Flow Cyt (Intra): Malme-3M cells. ICC/IF: Malme-3M cells
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
C5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12039の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 52-56 kDa (predicted molecular weight: 59 kDa).
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Flow Cyt (Intra) |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52-56 kDa (predicted molecular weight: 59 kDa). |
Flow Cyt (Intra)
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. -
組織特異性
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. -
関連疾患
Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete. -
配列類似性
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4286 Human
- Entrez Gene: 17342 Mouse
- Entrez Gene: 25094 Rat
- Omim: 156845 Human
- SwissProt: O75030 Human
- SwissProt: Q08874 Mouse
- SwissProt: O88368 Rat
- Unigene: 166017 Human
see all -
別名
- BHLHE32 antibody
- bHLHe32 antibody
- Class E basic helix-loop-helix protein 32 antibody
see all
画像
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ab12039 staining MiTF in Malme-3M cells. The cells were permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab12039 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Anti-MiTF antibody [C5] (ab12039) at 1 µg/ml + Malme 3m (Human melanoma cells) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 40,55,70 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes -
Overlay histogram showing Malme 3m cells stained with ab12039 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12039, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (102)
ab12039 は 102 報の論文で使用されています。
- Wang J et al. The combination of sucrose dilaurate and sucrose laurate suppresses HMGB1: an enhancer of melanocyte dendricity and melanosome transfer to keratinocytes. J Eur Acad Dermatol Venereol 36 Suppl 3:3-11 (2022). PubMed: 35014730
- Estrada C et al. MITF activity is regulated by a direct interaction with RAF proteins in melanoma cells. Commun Biol 5:101 (2022). PubMed: 35091687
- Pustovalova M et al. CD44+ and CD133+ Non-Small Cell Lung Cancer Cells Exhibit DNA Damage Response Pathways and Dormant Polyploid Giant Cancer Cell Enrichment Relating to Their p53 Status. Int J Mol Sci 23:N/A (2022). PubMed: 35563313
- Sultan F et al. Temporal analysis of melanogenesis identifies fatty acid metabolism as key skin pigment regulator. PLoS Biol 20:e3001634 (2022). PubMed: 35584084
- Chauvistré H et al. Persister state-directed transitioning and vulnerability in melanoma. Nat Commun 13:3055 (2022). PubMed: 35650266