Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Sample
Mouse Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
45 minute(s) · Temperature: 20°C · Diluent: Antibody Diluent (Dako)
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP polymer (pre-diluted, Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Specifically expressed in melanin-containing basal cells (arrowed).
Abcam response
We would like to thank this user for their valuable feedback. Unfortunately, we believe the staining pattern shown is not as it would be expected. This antibody was tested in-house in IHC and it was determined to not be suitable for IHC.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
投稿 Apr 07 2009