Anti-Mint3 抗体 (ab3450)
Key features and details
- Rabbit polyclonal to Mint3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Mint3 antibody
Mint3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Mint3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat Mint3 aa 1-19.
Sequence:MEFLPEPQHPPGPPTMDLE
(Peptide available asab4981) -
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to munc-18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint 3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint 3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 61 kDa). By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract. |
ターゲット情報
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機能
May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN. -
組織特異性
Expressed in all tissues examined with lower levels in brain and testis. -
配列類似性
Contains 2 PDZ (DHR) domains.
Contains 1 PID domain. -
ドメイン
Composed of an N-terminal domain, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane. -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 9546 Human
- Entrez Gene: 57267 Mouse
- Entrez Gene: 83611 Rat
- Omim: 604262 Human
- SwissProt: O96018 Human
- SwissProt: O88888 Mouse
- SwissProt: O70248 Rat
- Unigene: 25527 Human
see all -
別名
- Adapter protein X11gamma antibody
- amyloid beta (A4) precursor protein binding, family A, member 3 antibody
- Amyloid beta A4 precursor protein-binding family A member 3 antibody
see all
画像
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Western blot - Anti-Mint3 antibody (ab3450)
Western blot detection of Mint3 on AtT20 cell extract using ab3450.
プロトコール
データシートおよび資料
参考文献 (0)
ab3450 は論文での使用が確認できていません。
