Anti-Mint3 抗体 (ab3450)

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Key features and details

  • Rabbit polyclonal to Mint3
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

製品の概要

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 一次抗体 備考

    The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to munc-18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint 3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint 3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 61 kDa).

By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract.

ターゲット情報

  • 機能

    May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN.

  • 組織特異性

    Expressed in all tissues examined with lower levels in brain and testis.

  • 配列類似性

    Contains 2 PDZ (DHR) domains.
    Contains 1 PID domain.

  • ドメイン

    Composed of an N-terminal domain, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.

  • 細胞内局在

    Cytoplasm > perinuclear region.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Adapter protein X11gamma antibody
    • amyloid beta (A4) precursor protein binding, family A, member 3 antibody
    • Amyloid beta A4 precursor protein-binding family A member 3 antibody
    • Amyloid beta precursor protein binding, family A, member 3 phosphotyrosine binding/ interacting domain (PTB) bearing protein antibody
    • APBA3 antibody
    • APBA3_HUMAN antibody
    • lin 10 antibody
    • MGC:15815 antibody
    • Mint 3 antibody
    • Mint-3 antibody
    • Neuron specific X11L2 protein antibody
    • Neuron-specific X11L2 protein antibody
    • Neuronal Munc18 1 interacting protein 3 antibody
    • Neuronal Munc18-1-interacting protein 3 antibody
    • phosphotyrosine binding/interacting domain (PTB) bearing protein antibody
    • X11 like 2 protein 2 antibody
    • X11gamma antibody
    • X11L2 antibody
    see all

画像

  • Western blot - Anti-Mint3 antibody (ab3450)
    Western blot - Anti-Mint3 antibody (ab3450)

    Western blot detection of Mint3 on AtT20 cell extract using ab3450.

参考文献 (0)

ab3450 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3450 は論文での使用が確認できていません。

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Mint3 antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Specification
MEF
Fixative
Methanol/Acetone 50:50
Permeabilization
Yes - PBS, Triton X-100 (0.1%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
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投稿 Jul 06 2010

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