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Anti-Milk Fat Globule 1 抗体 [SPM291] (ab17787)

Reviews (1)Q&A (5)References (6)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)

Key features and details

  • Mouse monoclonal [SPM291] to Milk Fat Globule 1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Milk Fat Globule 1 antibody [SPM291]
    Milk Fat Globule 1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [SPM291] to Milk Fat Globule 1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    The details of the immunogen for this antibody are not available.

  • ポジティブ･コントロール

    • IHC: Human breast carcinoma, renal cell carcinoma, pancreas and lung carcinoma tissue

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab17787の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
    Medin is the main constituent of aortic medial amyloid.

  • 組織特異性

    Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.

  • 配列類似性

    Contains 1 EGF-like domain.
    Contains 2 F5/8 type C domains.

  • 翻訳後修飾

    Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.

  • 細胞内局在

    Membrane. Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BA46 antibody
      • Breast epithelial antigen BA46 antibody
      • EDIL1 antibody
      • HMFG antibody
      • hP47 antibody
      • HsT19888 antibody
      • Lactadherin antibody
      • Medin antibody
      • MFG-E8 antibody
      • MFGE8 antibody
      • MFGM antibody
      • MFGM_HUMAN antibody
      • Milk fat globule EGF factor 8 antibody
      • Milk fat globule EGF factor 8 protein antibody
      • Milk fat globule-EGF factor 8 antibody
      • O acetyl disialoganglioside synthase antibody
      • OAcGD3S antibody
      • SED1 antibody
      • SPAG10 antibody
      • Sperm associated antigen 10 antibody
      • Sperm surface protein hP47 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)

      IHC image of ab17787 staining in human carinoma Renal Cell formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)

      IHC image of ab17787 staining in human carinoma breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Milk Fat Globule 1 with ab18878 at 1 ug/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling Milk Fat Globule 1 with ab18878 at 1 ug/ml.

    参考文献 (6)

    ab17787 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab17787 は 6 報の論文で使用されています。

    • Xiao L  et al. Regulation of striatal cells and goal-directed behavior by cerebellar outputs. Nat Commun 9:3133 (2018). PubMed: 30087345
    • Baumgartner M  et al. Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self-amplifying radial glial cells. Development 145:N/A (2018). PubMed: 30093551
    • Yu L  et al. MFGE8 regulates TGF-ß-induced epithelial mesenchymal transition in endometrial epithelial cells in vitro. Reproduction 152:225-33 (2016). ICC/IF, WB ; Human . PubMed: 27340235
    • Soki FN  et al. Polarization of Prostate Cancer-associated Macrophages Is Induced by Milk Fat Globule-EGF Factor 8 (MFG-E8)-mediated Efferocytosis. J Biol Chem 289:24560-72 (2014). IHC-P ; Human . PubMed: 25006249
    • Yu L  et al. Tumor necrosis factor a up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor ?B activation, resulting in cell migration of epithelial cells. Fertil Steril N/A:N/A (2013). Human . PubMed: 24262600
    • Franchi A  et al. Expression of milk fat globule EGF-factor 8 (MFG-E8) mRNA and protein in the human endometrium and its regulation by prolactin. Mol Hum Reprod 17:360-71 (2011). WB, ICC/IF, IHC-P, IHC-Fr ; Human . PubMed: 21177637

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    Western blot abreview for Anti-Milk Fat Globule 1 antibody [SPM291]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (MCF7 cells)
    Specification
    MCF7 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Abcam user community

    Verified customer

    投稿 Jan 07 2015

    Question

    no bands on western blot from cell extract that, via mass spec, had shown expression of target protein

    Read More
    Answer


    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.
    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.
    The credit note ID is for your reference only and does not automatically guarantee the credit.
    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Question

    I’m in AB4587 and don’t see a link to MSDS.  I see the Data sheet link to the right, but no link to msds. Could you look at this page and tell me where it is?   https://www.abcam.com/Alzheimer-brain-human-temporal-lobe-tissue-slides-ab4587.html    

    Read More
    Answer

    Thanks for your email. ab4587 does not have any required MSDS information, so a link will not be present on this datasheet. For example, ab7254 does have a link to MSDS information. I hope this helps. Please contact me again if you have any further questions. 

    Read More

    Question

        I’m looking for MSDS for the items below. B-Amyloid Control Slides (5 Slides/PK) ABCAM AB4587 BCL2 Antibody ABCAM AB32124 BETA Amyloid ABCAM   BLIMP-1 ABCAM AB81961 FOXP3 ANTIBODY (236A/E7) ABCAM WIHC031 GCET1 ABCAM AB68889 GPNMB Antibody (Monoclonal) (100ug) ABCAM AB56584 Human Milk Fat Globule 1 Antibody  (500 uL) ABCAM AB17787 IMP-3 antibody ABCAM AB82044 Mesothelioma Positive Controls ABCAM AB4437 Oct1 Antibody (500uL) ABCAM AB15112 SMOOTHELIN ANTIBODY ABCAM   TFE-3 ABCAM AB70008 Ubiquitin ABCAM AB7254      

    Read More
    Answer

    Thanks for your enquiry. If required, MSDS information is located via one or more 'MSDS' links near the top left corner of the online datasheet. This link is just below the 'Datasheet PDF' link present on all datasheets. I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Question

    A t-on un anti hMFG1 qui reconnaitrait aussi le hMFG8 sur une sequence commune aux deux proteines?

    Read More
    Answer

    Nos anticorps anti hMFG1 sont des anticorps monoclonaux contre la protéine totale hMFG1, et nous ne savons pas ou se situe l'épitope. Cependant, j'ai effectué une recherche d'homologie entre les formes 1 et 8 et vous trouverez en attachement les résultats de celle-ci. La forme hMFG1 est composée de 335 acides aminés, la forme hMFG8 de ces même 335 acides aminés, plus une partie non homologue, ce qui forme au total une protéine de 387 acides aminés. Comme les anticorps monoclonaux sont en fait contre une partie identique aux deux protéines, nous pouvons prédire qu'ils réagiront également contre hMFG8. Comme cela n'a pas été testé je ne peux pas vous le garantir, mais j'espère que ces données vous aiderons dans votre recherche.

    Read More

    Question

    Hi, I am interested in purchasing an anti-lactadherin antibody suitable to carry out immunohistochemistry, but the antibody you supply ab17787 binds to an unknown human milk fat globule antigen. This is as it is raised against delipidated HMFG membrane. I wondered therefore if it would be possible for you to let me have a small quantity free of charge so that I could determine whether it does bind to lactadherin by western blot, as I could not afford to purchase an antibody that proved tot to bind.

    Read More
    Answer

    Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. We do, however, have our Abreview system, where researchers can submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for rewards such as Amazon.com gift certificates or discounts on future antibody purchases. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. In addition, our Abpromise states that if the antibody does not perform as described on this datasheet, notify us within 90 days of delivery, so that we can help you or offer you a replacement or a refund. The datasheet states that the antibody will recognize lactadherin by Western blot, so if you do have a problem please contact us. If we cannot troubleshoot your problem, we will offer you a replacement, credit note, or refund. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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