Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (immortalised bone marrow-derived macrophages)
Specification
immortalised bone marrow-derived macrophages
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/250
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% goat serum/3% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa-Fluor 488 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Cells were counterstained with TRIC-phalloidin (red)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. James Harris
Verified customer
投稿 Aug 24 2012