Key features and details
- Rabbit polyclonal to MIF
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.1% Sodium azide
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab7207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.|
機能Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
関連疾患Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
配列類似性Belongs to the MIF family.
細胞内局在Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
- Information by UniProt
- GIF antibody
- GLIF antibody
- Glycosylation inhibiting factor antibody
Anti-MIF antibody (ab7207) + LPS stimulated RAW cell lysate
Performed under reducing conditions.
Predicted band size: 12 kDa
MIF detected by Western Blot in LPS stimulated RAW cell lysate.
All lanes : Anti-MIF antibody (ab7207) at 1/1000 dilution
Lanes 1-2 : Whole cell lysate prepared from normal rat serum
Lanes 3-4 : Whole cell lysate prepared from rat serum (90 minutes warm ischemia and 1h reperfusion)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
From a review by Arsi Rosengren.
ab7207 は 27 報の論文で使用されています。
- Ha W et al. Ibudilast sensitizes glioblastoma to temozolomide by targeting Macrophage Migration Inhibitory Factor (MIF). Sci Rep 9:2905 (2019). PubMed: 30814573
- Shveiky D et al. Age-associated impairments in tissue strength and immune response in a rat vaginal injury model. Int Urogynecol J N/A:N/A (2019). PubMed: 31243497
- Ussher JR et al. Inactivation of the Glucose-Dependent Insulinotropic Polypeptide Receptor Improves Outcomes following Experimental Myocardial Infarction. Cell Metab 27:450-460.e6 (2018). PubMed: 29275960
- Hyrenius-Wittsten A et al. De novo activating mutations drive clonal evolution and enhance clonal fitness in KMT2A-rearranged leukemia. Nat Commun 9:1770 (2018). PubMed: 29720585
- Lang T et al. Macrophage migration inhibitory factor is required for NLRP3 inflammasome activation. Nat Commun 9:2223 (2018). PubMed: 29884801