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Anti-MIEF2 抗体 (ab101350)

Reviews (1) Submit a question References (1)
Western blot - Anti-MIEF2 antibody (ab101350)

    Key features and details

    • Rabbit polyclonal to MIEF2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-MIEF2 antibody

    • 製品の詳細

      Rabbit polyclonal to MIEF2

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Recombinant fragment corresponding to Human MIEF2 aa 299-441. (AAH14973)

    • ポジティブ･コントロール

      • 293T, A431, Jurkat and Raji whole cell lysates.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab101350の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    1/500 - 1/3000. Predicted molecular weight: 49 kDa.
    特記事項
    WB
    1/500 - 1/3000. Predicted molecular weight: 49 kDa.

    ターゲット情報

    • 機能

      Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity.

    • 組織特異性

      Expressed in all tissues tested with highest expression in heart and skeletal muscle.

    • 配列類似性

      Belongs to the MID49/MID51 family.

    • 細胞内局在

      Mitochondrion outer membrane. Colocalizes with DNM1L at mitochondrial membrane. Forms foci and rings around mitochondria.
    • Information by UniProt

    • 参照データベース

      • 別名

        • MGC23130 antibody
        • MID49 antibody
        • MID49_HUMAN antibody
        • MIEF2 antibody
        • Mitochondrial dynamic protein MID49 antibody
        • Mitochondrial dynamic protein of 49 kDa antibody
        • Mitochondrial dynamics protein MID49 antibody
        • Mitochondrial dynamics protein of 49 kDa antibody
        • Mitochondrial elongation factor 2 antibody
        • Smith Magenis syndrome chromosome region candidate 7 antibody
        • Smith-Magenis syndrome chromosomal region candidate gene 7 protein antibody
        see all

      画像

      • Western blot - Anti-MIEF2 antibody (ab101350)
        Western blot - Anti-MIEF2 antibody (ab101350)
        Anti-MIEF2 antibody (ab101350) at 1/500 dilution + Raji whole cell lysate at 30 µg

        Predicted band size: 49 kDa



        10% SDS PAGE

      参考文献 (1)

      ab101350 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab101350 は 1 報の論文で使用されています。

      • Li H  et al. Mst1 deletion attenuates renal ischaemia-reperfusion injury: The role of microtubule cytoskeleton dynamics, mitochondrial fission and the GSK3ß-p53 signalling pathway. Redox Biol 20:261-274 (2019). PubMed: 30384260

      レビューと Q&A

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      Western blot abreview for Anti-SMCR7 antibody

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      30 µg
      Gel Running Conditions
      Reduced Denaturing (4-12)
      Sample
      Rat Cell lysate - whole cell (Primary Neurons)
      Specification
      Primary Neurons
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Sep 05 2013

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