Anti-MICU2 抗体 (ab101465)
Key features and details
- Rabbit polyclonal to MICU2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MICU2 antibody -
製品の詳細
Rabbit polyclonal to MICU2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human MICU2 aa 95-238. (AAH31089)
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ポジティブ･コントロール
- U87-MG whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101465の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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配列類似性
Contains 4 EF-hand domains. - Information by UniProt
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参照データベース
- Entrez Gene: 221154 Human
- Entrez Gene: 68514 Mouse
- Omim: 610632 Human
- SwissProt: Q8IYU8 Human
- SwissProt: Q8CD10 Mouse
- Unigene: 412103 Human
- Unigene: 26834 Mouse
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別名
- 1110008L20Rik antibody
- EF hand domain containing family member A1 antibody
- EF hand domain family A1 antibody
see all
画像
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Western blot - Anti-MICU2 antibody (ab101465)Anti-MICU2 antibody (ab101465) at 1/1000 dilution + U87-MG whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU2 antibody (ab101465)Immunohistochemical analysis of FFPE mouse kidney tissue, labeling MICU2 with ab101465 (diluted 1/500).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU2 antibody (ab101465)Immunohistochemical analysis of FFPE human hepatoma tissue, labeling MICU2 with ab101465 (diluted 1/500).
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Immunocytochemistry/ Immunofluorescence - Anti-MICU2 antibody (ab101465)Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed MCF-7 cell line, labeling MICU2 with ab101465 (diluted 1/200). DNA stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab101465 は 24 報の論文で使用されています。
- Vishnu N et al. Mitochondrial clearance of calcium facilitated by MICU2 controls insulin secretion. Mol Metab 51:101239 (2021). PubMed: 33932586
- Wu R et al. MCU That Is Transcriptionally Regulated by Nrf2 Augments Malignant Biological Behaviors in Oral Squamous Cell Carcinoma Cells. Biomed Res Int 2021:6650791 (2021). PubMed: 34189138
- Cupo RR & Shorter J Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations. Elife 9:N/A (2020). PubMed: 32573439
- Plotegher N et al. Impaired cellular bioenergetics caused by GBA1 depletion sensitizes neurons to calcium overload. Cell Death Differ 27:1588-1603 (2020). PubMed: 31685979
- Calvo-Rodriguez M et al. Increased mitochondrial calcium levels associated with neuronal death in a mouse model of Alzheimer's disease. Nat Commun 11:2146 (2020). PubMed: 32358564