Anti-MIA3/TANGO1 抗体 (ab97881)
Key features and details
- Rabbit polyclonal to MIA3/TANGO1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MIA3/TANGO1 antibody
MIA3/TANGO1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MIA3/TANGO1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow
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免疫原
Recombinant fragment corresponding to Human MIA3/TANGO1 aa 1-120.
Database link: XP_496436 -
ポジティブ･コントロール
- A431, H1299, HeLa and HepG2 cell lines
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97881の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 214 kDa.
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| ICC/IF |
1/100 - 1/200.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 214 kDa. |
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ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. -
組織特異性
Broadly expressed, except in bone marrow and peripheral blood mononuclear cells. Down-regulated in melanoma tissue. -
配列類似性
Belongs to the MIA/OTOR family. Tango1 subfamily.
Contains 1 SH3 domain. -
ドメイン
The proline-rich region (PRD) mediates the interaction with COPII coat subunits Sec23/24.
Although 2 transmembrane domains are predicted, PubMed:19269366 showed that it only contains one transmembrane domain. The other predicted transmembrane region is probably a hairpin-type region embedded into the membrane, which does not cross the membrane. It is unclear which of the 2 predicted transmembrane regions is the transmembrane or the hairpin-type region. -
細胞内局在
Endoplasmic reticulum membrane. Localizes at endoplasmic reticulum exit sites. After loading of COL7A1 into transport carriers, it is not incorporated into COPII carriers and remains in the endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 375056 Human
- Omim: 613455 Human
- SwissProt: Q5JRA6 Human
- Unigene: 118474 Human
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別名
- ARNT antibody
- C219 reactive peptide antibody
- C219-reactive peptide antibody
see all
画像
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Western blot - Anti-MIA3/TANGO1 antibody (ab97881)All lanes : Anti-MIA3/TANGO1 antibody (ab97881) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : Hep G2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 214 kDa5% SDS PAGE.
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Immunocytochemistry/ Immunofluorescence - Anti-MIA3/TANGO1 antibody (ab97881)Immunofluorescence analysis of methanol fixed HepG2 cells, using ab97881 at a 1/200 dilution.
Lower image: costained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab97881 は 2 報の論文で使用されています。
- Du Q et al. Exosomal miR-30a and miR-222 derived from colon cancer mesenchymal stem cells promote the tumorigenicity of colon cancer through targeting MIA3. J Gastrointest Oncol 12:52-68 (2021). PubMed: 33708424
- Abou-Antoun TJ et al. Molecular and functional analysis of anchorage independent, treatment-evasive neuroblastoma tumorspheres with enhanced malignant properties: A possible explanation for radio-therapy resistance. PLoS One 13:e0189711 (2018). PubMed: 29298329