Anti-MHC class I + HLA A + HLA B 抗体 [EPR1394Y] (ab134189)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1394Y] to MHC class I + HLA A + HLA B
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y]
MHC class I+HLA A+HLA B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1394Y] to MHC class I + HLA A + HLA B -
由来種
Rabbit -
特異性
In our hands this antibody did not work in WB with mouse samples, but customer data suggest that reactivity in ICC/IF and IHC. The target shows high sequence homology with HLA-C and HLA-E, and it is possible that this antibody will detect these proteins too.
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アプリケーション
適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human MHC class I. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Raji, THP-1, and U-937 whole cell lysates. Human liver tissue lysate; ICC/IF: Raji cells; IHC-P: Human colon carcinoma tissue; Flow Cyt: Raji cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1394Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab134189の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
For unpurified use at 1/1000 - 1/10000. |
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IHC-P | (2) |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt |
1/100.
For unpurified use at 1/20 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/500.
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特記事項 |
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WB
1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
1/100. For unpurified use at 1/20 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/500. |
ターゲット情報
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細胞内局在
MHC class I: Cell Membrane; Type I membrane protein. HLA B: Membrane. -
参照データベース
- Entrez Gene: 3105 Human
- Entrez Gene: 3106 Human
- Omim: 142800 Human
- Omim: 142830 Human
- SwissProt: P01889 Human
- SwissProt: P03989 Human
- SwissProt: P04439 Human
- SwissProt: P30443 Human
see all
画像
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Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells, labeling MHC class I+HLA A+HLA B with Purified ab134189 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with / and permeabilised with /. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling MHC class I+HLA A+HLA B with purified ab134189 at 1/4000 dilution (0.26 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
All lanes : Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189) at 1/2000 dilution (Purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Human liver lysate
Lane 3 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 4 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.Blocking/Diluting buffer: 5% NFDM/TBST
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Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling MHC class I+HLA A+HLA B with purified ab134189 at 1/500 dilution (4.3 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab134189 は 17 報の論文で使用されています。
- Ahn JY et al. Effect of Human Peripheral Blood Mononuclear Cells on Mouse Endometrial Cell Proliferation: A Potential Therapeutics for Endometrial Regeneration. Gynecol Obstet Invest 87:105-115 (2022). PubMed: 35350012
- Zheng G et al. NCAPG Promotes Tumor Progression and Modulates Immune Cell Infiltration in Glioma. Front Oncol 12:770628 (2022). PubMed: 35372056
- Chen T et al. Implication of COPB2 Expression on Cutaneous Squamous Cell Carcinoma Pathogenesis. Cancers (Basel) 14:N/A (2022). PubMed: 35454945
- Manon J et al. Periosteum and fascia lata: Are they so different? Front Bioeng Biotechnol 10:944828 (2022). PubMed: 36338112
- Yao H et al. A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions. RSC Chem Biol 2:192-205 (2021). PubMed: 34458782