Anti-MHC class I 抗体 [R1-21.2] - Rabbit IgG (Chimeric) (ab281902)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [R1-21.2] to MHC class I - Chimeric
- Suitable for: ICC, WB
- Reacts with: Mouse
製品の概要
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製品名
Anti-MHC class I antibody [R1-21.2] - Rabbit IgG (Chimeric)
MHC class I 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [R1-21.2] to MHC class I - Chimeric -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse MHC class I. Raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
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ポジティブ・コントロール
- WB: Mouse thymus tissue lysate. ICC: Mouse splenocytes cells.
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特記事項
ab281902 is the chimeric rabbit version of ab281904.
This rabbit monoclonal chimeric antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
R1-21.2 -
アイソタイプ
IgG -
軽鎖の種類
kappa -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab281902の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa.
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特記事項 |
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ICC
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. |
ターゲット情報
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関連性
MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen. -
細胞内局在
Cell Membrane; Type I membrane protein. -
別名
- A 28 antibody
- A 9 antibody
- Antigen presenting molecule antibody
see all
画像
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Anti-MHC class I antibody [R1-21.2] - Rabbit IgG (Chimeric) (ab281902) at 1 µg/ml + Mouse thymus tissue lysate at 35 µg
Secondary
Anti-rabbit secondary antibody
Predicted band size: 41 kDaPrimary incubation of 1h was used and protein was detected by chemiluminescence.
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Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes labelling MHC-I with ab281902 at 10 µg/ml, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/ top-left). Image showing membrane staining in a subset of cells. The nuclear counter stain is DAPI (blue). The isotype control was anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody (bottom-right). Mouse splenocytes were immobilized on Shi-fix™ cover-slips.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab281902 は 1 報の論文で使用されています。
- Wu X et al. WEE1 inhibitor and ataxia telangiectasia and RAD3-related inhibitor trigger stimulator of interferon gene-dependent immune response and enhance tumor treatment efficacy through programmed death-ligand 1 blockade. Cancer Sci 112:4444-4456 (2021). PubMed: 34382294