Anti-MHC class I 抗体 [OX18] (ab6405)

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Key features and details

  • Mouse monoclonal [OX18] to MHC class I
  • Suitable for: IHC-Fr, Flow Cyt
  • Reacts with: Rat
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-MHC class I antibody [OX18]
    MHC class I 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [OX18] to MHC class I

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Fr, Flow Cytmore details

  • 種交差性

    交差種: Rat

  • 免疫原

    Other Immunogen Type corresponding to MHC class I. Rat spleen membrane glycoproteins

  • ポジティブ･コントロール

    • IHC-Fr: Normal rat placenta tissue. Flow Cyt: Lewis rat splenocytes.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    OX18

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 1 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.

  • 細胞内局在

    Cell Membrane; Type I membrane protein.

  • 別名

    • A 28 antibody
    • A 9 antibody
    • Antigen presenting molecule antibody
    • Aw 24 antibody
    • Aw 68 antibody
    • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
    • H2 K1 antibody
    • H2K antibody
    • HLA A antibody
    • HLA class I histocompatibility antigen A 1 alpha chain antibody
    • HLA class I histocompatibility antigen A 11 alpha chain antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A 24 alpha chain antibody
    • HLA class I histocompatibility antigen A 3 alpha chain antibody
    • HLA class I histocompatibility antigen A 30 alpha chain antibody
    • HLA class I histocompatibility antigen A 32 alpha chain antibody
    • HLA class I histocompatibility antigen A 68 alpha chain antibody
    • HLAA antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • MHC class I antigen A 1 antibody
    • MHC class I antigen A*11 antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A*24 antibody
    • MHC class I antigen A*3 antibody
    • MHC class I antigen A*30 antibody
    • MHC class I antigen A*32 antibody
    • MHC class I antigen A*68 antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC class I heavy chain H2 K antibody
    see all

画像

  • Flow Cytometry - Anti-MHC class I antibody [OX18] (ab6405)
    Flow Cytometry - Anti-MHC class I antibody [OX18] (ab6405)

    ab170190) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6405) or mouse IgG1κ (ab170190) isotype (1x 10ab150117) was used at 1/2000 dilution for 30 min on ice.

  • Immunohistochemistry (Frozen sections) - Anti-MHC class I antibody [OX18] (ab6405)
    Immunohistochemistry (Frozen sections) - Anti-MHC class I antibody [OX18] (ab6405)

    IHC image of MHC Class I staining in a section of frozen normal rat placenta*.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab6405 (shown in green) at 1 μg/ml. The section was then incubated with ab150117 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488, 1/1000)) presabsorbed for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount® .

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

    *Tissue obtained from Charles River.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab6405 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6405 は論文での使用が確認できていません。

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Question

Has this antibody been used with formalin-fixed paraffin-embedded tissue?

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Answer

No, this antibody has not been tested on formalin-fixed paraffin-embedded tissue

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Question

Hi, BD pharmingen sells OX-18. They say it works in most nucleated cells except normal neurons and glial cells. You say OX-10 works in all haemopoietic cells and 30% of normal thymocytes. What's the scoop do you know of any publications that back up what you say? Are these the same clones? Michele

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Answer

The only reference we have is Fukmoto T, McMaster WR, Williams AF (1982), Eur. J. Immunol. 12: 237-243. I have checked the information we received from the source of this product which confirms that OX-18 works in all haemopoietic cells and 30% of normal thymocytes. As both clones are OX-18, it is likely that they are from the same original source

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Question

Hello~! So I would like to know cross reactive of this Ab. Do this Ab react with MHC class 1 of mouse ?

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Answer

As far as we know, this Ab has not been tested for reactivity with the mouse antigen.

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