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  1. Link

    mh2a2-antibody-ab102126.pdf

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Epigenetics and Nuclear Signaling Histones Variants
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Anti-mH2A2 抗体 (ab102126)

  • Datasheet
  • SDS
Reviews (3)Q&A (3)References (2)

Product price, shipping and contact information

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Western blot - Anti-mH2A2 antibody (ab102126)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (ab102126)

Key features and details

  • Rabbit polyclonal to mH2A2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human mH2A2 protein (ab131967)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一次抗体
Product image
Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898)

関連製品

医薬用外劇物

製品の概要

  • 製品名

    Anti-mH2A2 antibody
    mH2A2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to mH2A2
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Recombinant fragment corresponding to Human mH2A2 aa 197-340.
    Database link: AAH16172

  • ポジティブ・コントロール

    • A431 whole cell lysate, TOV-21G xenograft
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Histones
    • Variants

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human mH2A2 protein (ab131967)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab102126の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P (1)
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)
特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)

ターゲット情報

  • 機能

    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
  • 配列類似性

    Contains 1 histone H2A domain.
    Contains 1 Macro domain.
  • 細胞内局在

    Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
  • Target information above from: UniProt accession Q9P0M6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 537167 Cow
    • Entrez Gene: 55506 Human
    • Entrez Gene: 361844 Rat
    • SwissProt: Q9P0M6 Human
    • Unigene: 499953 Human
    • 別名

      • Core histone macro H2A2.2 antibody
      • Core histone macro-H2A.2 antibody
      • Core histone macroH2A2.2 antibody
      • H2A histone family member Y2 antibody
      • H2AFY2 antibody
      • H2AW_HUMAN antibody
      • Histone macroH2A2 antibody
      • Macro H2A.2 antibody
      • Macro H2A2 antibody
      • MacroH2A.2 antibody
      • MacroH2A2 antibody
      • mH2A2 antibody
      see all

    画像

    • Western blot - Anti-mH2A2 antibody (ab102126)
      Western blot - Anti-mH2A2 antibody (ab102126)
      Anti-mH2A2 antibody (ab102126) at 1/1000 dilution + A431 whole cell lysate at 30 µg

      Predicted band size: 40 kDa



      10% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (ab102126)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (ab102126)

      Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using ab102126 at 1/100 dilution.

    プロトコール

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (2)

    ab102126 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab102126 は 2 報の論文で使用されています。

    • Xu X  et al. The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formation. Nat Commun 12:3520 (2021). PubMed: 34112784
    • Maharana S  et al. Chromosome intermingling-the physical basis of chromosome organization in differentiated cells. Nucleic Acids Res 44:5148-60 (2016). PubMed: 26939888

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mH2A2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 28 2019

    ChIP abreview for Anti-mH2A2 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Tissue lysate - nuclear (Brain nuclei)
    Negative control
    IgG
    Specification
    Brain nuclei
    Detection step
    Other
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 5 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 0.5% Fromaldehyde
    Positive control
    H3
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 10 2017

    ChIP abreview for Anti-mH2A2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Cell lysate - whole cell (ES)
    Negative control
    macroH2a2-/- ES cells IgG ab
    Specification
    ES
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Positive control
    No
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 09 2011

    Question

    Dear Dr. Schulte:Thanks for the information, I hope you can identify this band soon, perhaps is a protein that shares homology with this region macroh2a2 (amino acids 197-340 of Human Macro H2A.2 (AAH16172)). Thank you very much, and expecting your response

    Read More

    Abcam community

    Verified customer

    Asked on Mar 01 2012

    Answer

    Thank you very much for your reply.

    All of the information we have for each of our products is listed on the online datasheet and these are updated as soon as we receive any new information. So once we know the identity of the band, you will find the information on the datasheet. Unfortunately, these kind of projects are not of a very high priority, especially as this additional band is clearly of a different size than the expected one.

    According to ourBLAST searches there is no other protein that shares significant homologywith this region of MacroH2A2. Given that thisband is observedwith acell line derived from a tumour, it might be that it isdue to the specificbiology of this cancer cell line A431.

    If you would like to use this antibody with different cells, we would be very grateful if you could submit an Abreview about this product via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.To find out more about our Abreview system, please see the following webpage: https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Mar 01 2012

    Question

    Dear Abcam plc/Inc: I have a question, I buy the antibody (Anti-Macro H2A.2 antibody (ab102126)), where appears one band at 40 kDa corresponds to macroh2a2, by western blot, but too appear another band between 72 and 55 kDa. What is it this band?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 29 2012

    Answer

    Thank you very much for your enquiry.

    Please accept my apologies for the long delay of my reply. I have been in contact with the source of this antibody regarding the additional band between 72 and 55 kDa. We suspect that this band may be due to alternative splicing. Unfortunately, the identity of the protein causing this band has not been determined further. The lab however confirmed that when testing this antibody in mouse brain samples this band is only very faint.

    Please be reassured that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (www.abcam.com/Abpromise).

    If the product is to be used in an untested application or species (including the predicted to react rat and cow), you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

    We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. I hope this information is nevertheless helpful for you. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Feb 29 2012

    Question

    I would like to test this antibody in mouse.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 28 2011

    Answer

    I am very pleased to hear you would like to accept our offer and test ab4173 & ab102126 in mouse samples. This code will be worth the price you pay for ab4173 & ab102126 and can be used with any single future primary antibody purchase before the expiration date. To redeem this offer, you must first submit your Abreview with your mouse data. Please include this code in the “Additional Comments” section so that we will know the review is for this promotion. The code will be active once the Abreview has been submitted. The code can be redeemed in the following ways: 1) Call to place your order by phone and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and remember to enter your promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Oct 28 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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