Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7935] to MGST1 - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-MGST1 antibody [EPR7935] - BSA and Azide free
MGST1 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7935] to MGST1 - BSA and Azide free
アプリケーション適用あり: ICC, WBmore details
適用なし: Flow Cyt,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human MGST1 aa 100-200 (C terminal). The exact sequence is proprietary.
ab248331 is the carrier-free version of ab129175 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248331 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Microsomal Glutathione S-transferase 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 5.40 x 10 -12 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).|
機能Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.
組織特異性Highly expressed in liver.
配列類似性Belongs to the MAPEG family.
翻訳後修飾Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.
細胞内局在Microsome. Mitochondrion outer membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- MGST1 antibody
- Glutathione S transferase 12 antibody
- GST12 antibody
All lanes : Anti-MGST1 antibody [EPR7935] (ab129175) at 1/1000 dilution
Lane 1 : Fetal liver tissue lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HT 1376 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
This data was developed using ab129175, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.