Anti-MGST1 抗体 [EPR7935] (ab129175)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7935] to MGST1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MGST1 antibody [EPR7935]
MGST1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7935] to MGST1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human MGST1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P10620 -
ポジティブ・コントロール
- Fetal liver lysate, HepG2 cells and cell lysate, K562 cell lysate, HT 1376 cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 5.40 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7935 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129175の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
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ICC/IF |
1/100 - 1/250.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa). |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity. -
組織特異性
Highly expressed in liver. -
配列類似性
Belongs to the MAPEG family. -
翻訳後修飾
Peroxynitrite induces nitration at Tyr-93 which activates the enzyme. -
細胞内局在
Microsome. Mitochondrion outer membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4257 Human
- Entrez Gene: 56615 Mouse
- Entrez Gene: 171341 Rat
- Omim: 138330 Human
- SwissProt: P10620 Human
- SwissProt: Q91VS7 Mouse
- SwissProt: P08011 Rat
- Unigene: 389700 Human
see all -
別名
- MGST1 antibody
- Glutathione S transferase 12 antibody
- GST12 antibody
see all
画像
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All lanes : Anti-MGST1 antibody [EPR7935] (ab129175) at 1/1000 dilution
Lane 1 : Fetal liver tissue lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HT 1376 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted? -
ab129175, at 1/100, staining MGST1 in HepG2 cells by Immunofluorescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab129175 は論文での使用が確認できていません。