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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-MGMT 抗体 [EPR4398] (ab108989)

Submit a review Q&A (1)
Western blot - Anti-MGMT antibody [EPR4398] (ab108989)
  • Western blot - Anti-MGMT antibody [EPR4398] (ab108989)
  • Immunoprecipitation - Anti-MGMT antibody [EPR4398] (ab108989)
  • Western blot - Anti-MGMT antibody [EPR4398] (ab108989)
  • Anti-MGMT antibody [EPR4398] (ab108989)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4398] to MGMT
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MGMT antibody [EPR4398]
    MGMT 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4398] to MGMT

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: ICC/IF or IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • MCF7 and Jurkat cell lysates

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108989の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).
IP
1/10 - 1/100.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).
IP
1/10 - 1/100.
追加情報
Is unsuitable for ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

  • 配列類似性

    Belongs to the MGMT family.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 6 O methylguanine DNA methyltransferase antibody
      • 6-O-methylguanine-DNA methyltransferase antibody
      • Agat antibody
      • AGT antibody
      • AI267024 antibody
      • EC 2.1.1.63 antibody
      • Methylated DNA protein cysteine methyltransferase antibody
      • Methylated-DNA--protein-cysteine methyltransferase antibody
      • Methylguanine DNA methyltransferase antibody
      • MGC107020 antibody
      • MGMT antibody
      • MGMT_HUMAN antibody
      • O 6 methylguanine DNA alkyltransferase antibody
      • O 6 methylguanine DNA methyltransferase antibody
      • O-6-methylguanine-DNA methyltransferase antibody
      • O-6-methylguanine-DNA-alkyltransferase antibody
      see all

    画像

    • Western blot - Anti-MGMT antibody [EPR4398] (ab108989)
      Western blot - Anti-MGMT antibody [EPR4398] (ab108989)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MGMT knockout HAP1 cell lysate (20 µg)
      Lane 3: MCF7 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108989 observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab108989 was shown to specifically react with MGMT when MGMT knockout samples were used. Wild-type and MGMT knockout samples were subjected to SDS-PAGE. ab108989 and ab8245 (loading control to MGMT) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-MGMT antibody [EPR4398] (ab108989)
      Western blot - Anti-MGMT antibody [EPR4398] (ab108989)
      All lanes : Anti-MGMT antibody [EPR4398] (ab108989) at 1/1000 dilution (purified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Predicted band size: 22 kDa
      Observed band size: 23 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunoprecipitation - Anti-MGMT antibody [EPR4398] (ab108989)
      Immunoprecipitation - Anti-MGMT antibody [EPR4398] (ab108989)

      ab108989 (purified) at 1/30 immunoprecipitating MGMT in 10 μg MCF7 (Lanes 1 and 2, observed at 23 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-MGMT antibody [EPR4398] (ab108989)
      Western blot - Anti-MGMT antibody [EPR4398] (ab108989)
      All lanes : Anti-MGMT antibody [EPR4398] (ab108989) at 1/1000 dilution (unpurified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 22 kDa
      Observed band size: 23 kDa why is the actual band size different from the predicted?

    • Anti-MGMT antibody [EPR4398] (ab108989)
      Anti-MGMT antibody [EPR4398] (ab108989)

    参考文献 (0)

    ab108989 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108989 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Many thanks for your reply. The answer was perfect.
    I was also wondering whether you have any information regarding the concentration of the particular Lot that I have?

    Read More
    Answer

    Thank you for your reply.

    ab108989 is atissue culture supernatant, not a purified antibody.
    Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet.

    The concentration estimate of antibodyin tissue culture supernatants is usuallyin the range of1 - 3 mg/ml.

    I am sorry I cannot provide an exact concentration on this occasion, but I hope this information is nevertheless helpful to you.
    If you have any further questions, please do not hesitate to contact us.

    Read More

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    For licensing inquiries, please contact partnerships@abcam.com