Anti-MGEA5/OGA 抗体 (ab105217)
Key features and details
- Rabbit polyclonal to MGEA5/OGA
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MGEA5/OGA antibody
MGEA5/OGA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MGEA5/OGA -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rabbit, Horse, Cow, Dog, Pig, Macaque monkey, Gorilla
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive signal in the following whole cell lysates: U87mg; JEG3; PDX1. ICC/IF: SKNSH cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105217の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa). |
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ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1. -
組織特異性
Ubiquitous. Shows highest expression in the brain, placenta and pancreas. -
配列類似性
Belongs to the glycosyl hydrolase 84 family. -
翻訳後修飾
Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity. -
細胞内局在
Cytoplasm and Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 538561 Cow
- Entrez Gene: 10724 Human
- Entrez Gene: 76055 Mouse
- Omim: 604039 Human
- SwissProt: O60502 Human
- SwissProt: Q9EQQ9 Mouse
- Unigene: 500842 Human
- Unigene: 122725 Mouse
see all -
別名
- Beta-hexosaminidase antibody
- Beta-N-acetylglucosaminidase antibody
- Beta-N-acetylhexosaminidase antibody
see all
画像
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Western blot - Anti-MGEA5/OGA antibody (ab105217)All lanes : Anti-MGEA5/OGA antibody (ab105217) at 1 µg
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MGEA5 knockout HAP1 whole cell lysate
Lane 3 : U20S whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 103 kDaLanes 1 - 4: Merged signal (red and green). Green - ab105217 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab105217 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in MGEA5 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and MGEA5 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab105217 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Anti-MGEA5/OGA antibody (ab105217)ab105217 staining MGEA5 in SKNSH cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab105217 at 1/900 dilution and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). The secondary antibody (shown in green) was ab150081 Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Western blot - Anti-MGEA5/OGA antibody (ab105217)All lanes : Anti-MGEA5/OGA antibody (ab105217) at 1 µg/ml
Lane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 3 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate
Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lane 5 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lane 6 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab105217 は 2 報の論文で使用されています。
- Ferron M et al. O-GlcNAc stimulation: A new metabolic approach to treat septic shock. Sci Rep 9:18751 (2019). PubMed: 31822776
- Huang L et al. O-GlcNAc-modified SNAP29 inhibits autophagy-mediated degradation via the disturbed SNAP29-STX17-VAMP8 complex and exacerbates myocardial injury in type I diabetic rats. Int J Mol Med 42:3278-3290 (2018). PubMed: 30221662
