Anti-MGA 抗体 [EPR19854] (ab214814)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19854] to MGA
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-MGA antibody [EPR19854]
MGA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19854] to MGA -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Mouse
非交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Wild-type mouse embryonic stem (ES) cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19854 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab214814の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 329 kDa (predicted molecular weight: 328 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 329 kDa (predicted molecular weight: 328 kDa). |
ターゲット情報
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関連性
MGA contains 1 basic helix-loop-helix (bHLH) domain and is a possible transcription factor. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 29808 Mouse
- SwissProt: A2AWL7 Mouse
- SwissProt: Q3TS36 Mouse
- SwissProt: Q9QXJ5 Mouse
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別名
- MAD5 antibody
- MAX dimerization protein 5 antibody
- MAX gene associated antibody
see all
画像
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All lanes : Anti-MGA antibody [EPR19854] (ab214814) at 1/1000 dilution
Lane 1 : Wild-type mESCs (mouse embryonic stem cells) whole cell lysate
Lane 2 : MGA knockout mESCs whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 328 kDa
Observed band size: 329 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and Diluting buffer and concentration: 5% NFDM/TBST
The lysates were kindly provided by an anonymous collaborator.
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Anti-MGA antibody [EPR19854] (ab214814) at 1/1000 dilution + ES-D3 (mouse blastocyst-derived embryonic stem cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Developed using the ECL technique.
Predicted band size: 328 kDa
Observed band size: 329 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and Diluting buffer and concentration: 5% NFDM/TBST
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
This blot was developed using a high sensitivity ECL substrate.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab214814 は論文での使用が確認できていません。