Anti-Methionine Aminopeptidase 2/p67 抗体 [EPR6886(B)] - BSA and Azide free (ab248071)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6886(B)] to Methionine Aminopeptidase 2/p67 - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] - BSA and Azide free
Methionine Aminopeptidase 2/p67 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6886(B)] to Methionine Aminopeptidase 2/p67 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248071 is the carrier-free version of ab124953.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6886(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248071の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa).
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
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機能
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. -
配列類似性
Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily. -
翻訳後修飾
Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding. -
細胞内局在
Cytoplasm. About 30% of expressed METAP2 associates with polysomes. - Information by UniProt
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参照データベース
- Entrez Gene: 10988 Human
- Entrez Gene: 56307 Mouse
- Entrez Gene: 64370 Rat
- Omim: 601870 Human
- SwissProt: P50579 Human
- SwissProt: O08663 Mouse
- SwissProt: P38062 Rat
- Unigene: 444986 Human
see all -
別名
- A930035J23Rik antibody
- AI047573 antibody
- AL024412 antibody
see all
画像
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All lanes : Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : 293T cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : C6 cell lysates
Lane 5 : RAW 264.7 cell lysates
Lane 6 : PC12 cell lysates
Lane 7 : NIH 3T3 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?This data was developed using ab124953, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248071 は論文での使用が確認できていません。