製品名Metformin hydrochloride, AMPK activator
AMPK activator. Antidiabetic and anticancer activity in vivo. Rapidly crosses the blood-brain barrier.
Also available in simple stock solutions (ab146725) - add 1 ml of water to get an exact, ready-to-use concentration.
体系名N,N-Dimethylimidodicarbonimidic diamide hydrochloride
保存方法Store at Room Temperature. The product can be stored for up to 12 months.
溶解性Soluble in water to 100 mM and in DMSO to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab1 staining HIF1α in MCF7 cells treated with metformin hydrochloride (ab120847), by ICC/IF. Decrease in HIF1α expression correlates with increased concentration of metformin hydrochloride, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120847 (metformin hydrochloride) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120847 は 4 報の論文で使用されています。
- Gong Y et al. Metformin Inhibits Tumor Metastasis through Suppressing Hsp90a Secretion in an AMPKa1-PKC? Dependent Manner. Cells 9:N/A (2020). PubMed: 31936169
- Soto-Acosta R et al. DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target. PLoS Pathog 13:e1006257 (2017). PubMed: 28384260
- Blackmore K et al. LKB1-AMPK modulates nutrient-induced changes in the mode of division of intestinal epithelial crypt cells in mice. Exp Biol Med (Maywood) 242:1490-1498 (2017). PubMed: 28766983
- Zhou Z et al. Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NF?B Pathway Suppression. J Diabetes Res 2016:4847812 (2016). PubMed: 27761470