Metformin hydrochloride, AMPK activator (ab120847)
Key features and details
- AMPK activator
- CAS Number: 1115-70-4
- Purity: > 99%
Soluble in water to 100 mM and in DMSO to 50 mM.
Sonication and heating in a water bath to approximately 45 °C are required to produce a clear solution in DMSO.
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Metformin hydrochloride, AMPK activator -
製品の詳細
AMPK activator -
生理活性の詳細
AMPK activator. Antidiabetic and anticancer activity in vivo. Rapidly crosses the blood-brain barrier.
Also available in simple stock solutions (ab146725) - add 1 ml of water to get an exact, ready-to-use concentration. -
精製度
> 99% -
CAS 番号
1115-70-4 -
構造式
製品の特性
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体系名
N,N-Dimethylimidodicarbonimidic diamide hydrochloride
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分子量
165.63 -
分子式
C4H11N5.HCl -
PubChem 登録番号
14219 -
保存方法
Store at Room Temperature. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 100 mM and in DMSO to 50 mM.
Sonication and heating in a water bath to approximately 45 °C are required to produce a clear solution in DMSO.
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
CN(C)C(=N)N=C(N)N.Cl -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120847の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120847, Metformin hydrochloride, AMPK activator
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ab1 staining HIF1α in MCF7 cells treated with metformin hydrochloride (ab120847), by ICC/IF. Decrease in HIF1α expression correlates with increased concentration of metformin hydrochloride, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120847 (metformin hydrochloride) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (10)
ab120847 は 10 報の論文で使用されています。
- Rai D et al. Evaluation of photoreceptor-directed fibroblasts derived from retinitis pigmentosa patients with defects in the EYS gene: a possible cost-effective cellular model for mechanism-oriented drug. Stem Cell Res Ther 13:157 (2022). PubMed: 35410372
- Farfan-Morales CN et al. The antiviral effect of metformin on zika and dengue virus infection. Sci Rep 11:8743 (2021). PubMed: 33888740
- Ruparelia AA et al. Metformin rescues muscle function in BAG3 myofibrillar myopathy models. Autophagy 17:2494-2510 (2021). PubMed: 33030392
- Kundu A et al. 14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation. J Biol Chem 295:1754-1766 (2020). PubMed: 31901078
- Zhao Y et al. NEAT1 regulates microtubule stabilization via FZD3/GSK3ß/P-tau pathway in SH-SY5Y cells and APP/PS1 mice. Aging (Albany NY) 12:23233-23250 (2020). PubMed: 33221742
- Gong Y et al. Metformin Inhibits Tumor Metastasis through Suppressing Hsp90a Secretion in an AMPKa1-PKC? Dependent Manner. Cells 9:N/A (2020). PubMed: 31936169
- Wimmer RA et al. Human blood vessel organoids as a model of diabetic vasculopathy. Nature 565:505-510 (2019). PubMed: 30651639
- Blackmore K et al. LKB1-AMPK modulates nutrient-induced changes in the mode of division of intestinal epithelial crypt cells in mice. Exp Biol Med (Maywood) 242:1490-1498 (2017). PubMed: 28766983
- Soto-Acosta R et al. DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target. PLoS Pathog 13:e1006257 (2017). PubMed: 28384260
- Zhou Z et al. Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NF?B Pathway Suppression. J Diabetes Res 2016:4847812 (2016). PubMed: 27761470