Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 抗体 [EPR8975] (ab150387)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8975] to Metabotropic Glutamate Receptor 2/MGLUR2 + Metabotropic Glutamate Receptor 3/MGLUR3
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975]
Metabotropic Glutamate Receptor 2/MGLUR2+Metabotropic Glutamate Receptor 3/MGLUR3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8975] to Metabotropic Glutamate Receptor 2/MGLUR2 + Metabotropic Glutamate Receptor 3/MGLUR3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: Mouse primary neurone cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR8975 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab150387の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/1800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at dilution between 1/100 to 1/250 |
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WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 95 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
1/70.
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特記事項 |
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IHC-P
1/1800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at dilution between 1/100 to 1/250 |
WB
1/10000 - 1/50000. Predicted molecular weight: 95 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
1/70. |
ターゲット情報
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細胞内局在
Metabotropic Glutamate Receptor 2/MGLUR2: Cell membrane. Metabotropic Glutamate Receptor 3/MGLUR3: Cell membrane. -
参照データベース
- Entrez Gene: 2912 Human
- Entrez Gene: 2913 Human
- Entrez Gene: 108068 Mouse
- Entrez Gene: 108069 Mouse
- Entrez Gene: 24415 Rat
- Entrez Gene: 24416 Rat
- Omim: 601115 Human
- Omim: 604099 Human
see all
画像
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All lanes : Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387) at 1/10000 dilution
Lane 1 : HEK-293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc/flag-tag, not boiled whole cell lysate
Lane 2 : HEK-293T (Human embryonic kidney epithelial cell) transfected with MGLUR2 (aa1-872) with myc/flag-tag, not boiled whole cell lysate
Lane 3 : HEK-293T (Human embryonic kidney epithelial cell) transfected with MGLUR3 (aa1-879) with myc/flag-tag, not boiled whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 250,95 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking buffer: 5% NFDM/TBST.
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Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387) at 1/10000 dilution (Purified) + Human brain lysate unboiled
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 95 kDa -
All lanes : Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387) at 1/10000 dilution (Purified)
Lane 1 : Mouse brain lysate boiled
Lane 2 : Mouse brain lysate unboiled
Lane 3 : Rat brain lysate boiled
Lane 4 : Rat brain lysate unboiled
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 95 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1:1800 (0.36 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1:1800 (0.36 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1:1800 (0.36 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Flow Cytometry analysis of Mouse primary neuron cells cells labelling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1:70 dilution (10 ?g/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry analysis of mouse primary neurone cells labelling MGLUR2 with ab150387 at 2 µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-MAP2 rabbit monoclonal antibody (ab11267) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Confocal image showing positive staining in mouse primary neuron cells.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab150387 は 8 報の論文で使用されています。
- Orlando R et al. Synergic action of L-acetylcarnitine and L-methylfolate in Mouse Models of Stress-Related Disorders and Human iPSC-Derived Dopaminergic Neurons. Front Pharmacol 13:913210 (2022). PubMed: 35721218
- Fan S et al. Ceftriaxone Suppresses Group II Metabotropic Glutamate Receptor Expression Contributing to Reversal of Recognition Memory Deficits of Amyloid Precursor Protein/Presenilin 1 AD Mice. Front Neurosci 16:905403 (2022). PubMed: 35860293
- Brant B et al. IQSEC2 mutation associated with epilepsy, intellectual disability, and autism results in hyperexcitability of patient-derived neurons and deficient synaptic transmission. Mol Psychiatry 26:7498-7508 (2021). PubMed: 34535765
- Habrian CH et al. Conformational pathway provides unique sensitivity to a synaptic mGluR. Nat Commun 10:5572 (2019). PubMed: 31804469
- Dean B et al. Changes in levels of cortical metabotropic glutamate 2 receptors with gender and suicide but not psychiatric diagnoses. J Affect Disord 244:80-84 (2019). PubMed: 30326345
- Rogers EJ et al. An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. Front Mol Neurosci 12:43 (2019). PubMed: 30842726
- Wang J et al. Metabotropic glutamate receptor subtype 2 is a cellular receptor for rabies virus. PLoS Pathog 14:e1007189 (2018). PubMed: 30028877
- Molosh AI et al. Panic results in unique molecular and network changes in the amygdala that facilitate fear responses. Mol Psychiatry N/A:N/A (2018). PubMed: 30108314