Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12 (Pheochromocytoma cell line))
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Treatment
Lane1 untreated Lane2 500μM CoCl2
Specification
PC12 (Pheochromocytoma cell line)
Blocking step
Block one p as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution
1/800
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: donkey anti-rabbit antibody-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL prime
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 145 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. K. A.
Verified customer
投稿 May 20 2015