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Anti-MERTK (phospho Y749 + Y753 + Y754) 抗体 (ab14921)

Submit a review Q&A (7)References (9)

Key features and details

  • Rabbit polyclonal to MERTK (phospho Y749 + Y753 + Y754)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-MERTK (phospho Y749 + Y753 + Y754) antibody
    MERTK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to MERTK (phospho Y749 + Y753 + Y754)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human MERTK aa 700-800 (phospho Y749 + Y753 + Y754).
    Database link: Q12866

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab14921の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    In case of filovirus infection, seems to function as a cell entry factor.

  • 組織特異性

    Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.

  • 関連疾患

    Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • c MER antibody
      • c mer proto oncogene tyrosine kinase antibody
      • c-mer antibody
      • cMER antibody
      • cmer protooncogene tyrosine kinase antibody
      • Eyk antibody
      • MER antibody
      • MER receptor tyrosine kinase antibody
      • MERK antibody
      • MERPEN antibody
      • Mertk antibody
      • MERTK c-mer proto-oncogene tyrosine kinase antibody
      • MERTK_HUMAN antibody
      • MGC133349 antibody
      • nmf12 antibody
      • Nyk antibody
      • Proto oncogene tyrosine protein kinase MER antibody
      • Proto oncogene tyrosine protein kinase MER precursor antibody
      • Proto-oncogene c-Mer antibody
      • Receptor tyrosine kinase MerTK antibody
      • RP38 antibody
      • STK kinase antibody
      • Tyrosine-protein kinase Mer antibody
      see all

    参考文献 (9)

    ab14921 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab14921 は 9 報の論文で使用されています。

    • Du W  et al. KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway. Cell Death Differ 28:1284-1300 (2021). PubMed: 33139930
    • Chung SH  et al. Structure-activity relationship of 7-aryl-2-anilino-pyrrolopyrimidines as Mer and Axl tyrosine kinase inhibitors. J Enzyme Inhib Med Chem 35:1822-1833 (2020). PubMed: 32972253
    • Nam SH  et al. Lysyl-tRNA synthetase-expressing colon spheroids induce M2 macrophage polarization to promote metastasis. J Clin Invest 128:5034-5055 (2018). PubMed: 30188867
    • Kariolis MS  et al. Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies. J Clin Invest 127:183-198 (2017). WB ; Human . PubMed: 27893463
    • Jung Y  et al. Endogenous GAS6 and Mer receptor signaling regulate prostate cancer stem cells in bone marrow. Oncotarget 7:25698-711 (2016). WB . PubMed: 27028863
    • Murase H  et al. TUDCA Promotes Phagocytosis by Retinal Pigment Epithelium via MerTK Activation. Invest Ophthalmol Vis Sci 56:2511-8 (2015). PubMed: 25804419
    • Fuma S  et al. Photobiomodulation with 670 nm light increased phagocytosis in human retinal pigment epithelial cells. Mol Vis 21:883-92 (2015). WB . PubMed: 26321863
    • Xie S  et al. Mer receptor tyrosine kinase is frequently overexpressed in human non-small cell lung cancer, confirming resistance to erlotinib. Oncotarget 6:9206-19 (2015). WB ; Human . PubMed: 25826078
    • Alciato F  et al. TNF-alpha, IL-6, and IL-1 expression is inhibited by GAS6 in monocytes/macrophages. J Leukoc Biol 87:869-75 (2010). WB ; Human . PubMed: 20103767

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Question

    The product was purchased on the 14th of november by customer Leif Strid. Your order number is 1203398 and the invoice number is 1362140-SAH002
    Best regards,

    Read More
    Answer

    Thank you for contacting us.

    The actual order number for ab14921 was 1197605. I have sent a new ab52968 with order number 1219455.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question

    The purchase order number is 111351.

    Read More
    Answer

    Unfortunately this order does not match with anything? Please check again?

    Could you provide the address and date when this antibody was purchased?

    Thanks!

    Read More

    Question

    Unfortunately I don't personally have a purchase number since the order was placed through the departments centralised ordering. But the lot number on the tube is GR2171-8. Best regards,

    Read More
    Answer

    Could you contact the purchasing department for purchase order number?

    Thanks!

    Read More

    Question

    Since it is the only antibody you have against phosphorylated Mer, would it be possible instead to get another antibody against Mer? So far I have been happy with the ab52968, would it be possible to get one of these instead?Best regards,

    Read More
    Answer

    I have requested a retest of this antibody. I will let you know the results when available.

    Could you provide the purchase order number of ab14921? The PO will help us to send the ab at the same address we sent before.

    Please note without the purchase order number we will not be able to process the order.

    Looking forward to hearing from you soon.

    Read More

    Question

    I am using the EA.hy926 cell line in my experiments, and I stimulate them with Gas6 to induce Mer-phosphorylation. i have now repeated the experiment without using a primary ab as a control and I am attaching the image to this mail. As you can see, without primary antibody I have no signal at all (same exposure settings in ECL development). I also detected total mer from the blot to show similar loading of Mer.
    Best regards,

    Read More
    Answer

    Thank you for your email.

    I am sorry to hear that the results weren't improved. I am happy to replace the antibody with a new one however as this is the only phospho specific antibody we have so there in no option for replacement unless you want a different ab.

    In order to know more about the quality of this antibody I have sent your experimental details to my colleague in laboratory. So would you like to wait for the results or you would like to have refund.

    Once agian I am sorry for the inconvenience caused. I will look forward to hearing from you soon.

    Read More

    Question

    I have not repeated the experiment without primary antibody, however the blot where total Mer was detected (in the attached image in the original inquiry) used the same secondary antibody (swine anti-rabbit from Dako) at the same dilution. In this blot no band of 130 kDa was seen (or any other unspecific bands either), so I can be quite confident that this band is due to a reaction of the anti-pMer antibody.
    I have not changed any other parameters of the experiment since these are quite standard settings we use and should not influence the behaviour of the detecting antibodies. And at these conditions I see that Mer is well solubilised in the lysates and migrates as expected at ca 180 kDa.

    Best regards,

    Read More
    Answer

    Thanks for the information.

    The results are very strange. I am sorry I could not find a good reason why the antibodies are showing difference. Technically, they should show the same band size. The MERTK protein is heavily glycosylated so 180 kDa band is possible. Could you tell me which cell line you are using?

    Could you redo the experiment without stripping the membrane and by including the no primary control?

    Many thanks for your cooperation in this case. I will look forward to hearing from you soon.

    Read More

    Question

    Lot number: 1292976
    Inquiry: Hi, I have had a problem with your antibody against phosphorylated MerTK in western blot, it seems to recognise the wrong protein. I have run (Gas-6/Protein S stimulated) cell lysates corresponding to 20 ug total protein (lysis buffer 25 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 10% glycerol, 1% Triton X-100 with protease and phosphatase inhibitors) under reducing conditions on a 4-15% gradient gel followed by blotting to a PVDF membrane. As blocking reagent I used 3% fish gelatine in 50 mM Tris pH 8, 150 mM NaCl, 0.1% Tween-20, 1 h RT. I incubated the blot with the above mentioned antibody for 1 h at RT diluted 1:1000 in blocking buffer followed by 4 washes with 50 mM Tris pH 8, 150 mM NaCl, 0.1% Tween-20. As a secondary antibody I used a swine anti-rabbit HRP conjugate from Dako, diluted 1:3000 in blocking buffer. Incubation time again 1 h RT. The membrane was washed 4 times as above and developed using ECL. After phosphoMer detection the blot was stripped using 0.4 M NaOH (12 min RT) after which total Mer was analysed using the RabMAb a b52968. When detecting phosphoMer I see a band of approx 130 kDa as opposed to a band of ca 180 kDa when detecting total Mer. This obviously can not be the same protein. Since the antibody against the phosphorylated Mer gives only one band I can assume it is specific, but against another protein. For details, see attached file. Since this antibody is of no use in Mer-studies I was wondering If I can get another antibody as a compensation?

    Read More
    Answer

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    I have read the details you have kindly provided and would like to ask if you have repeated the experiment by changing any step in protocol. Did you include no primary control?

    Thank you very much for your cooperation. I will look forward to hearing from you soon.

    Read More

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