遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-MERTK 抗体 [Y323] - BSA and Azide free (ab271851)

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Immunoprecipitation - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)
  • Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y323] to MERTK - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Knockout validated
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-MERTK antibody [Y323] - BSA and Azide free
    MERTK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [Y323] to MERTK - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human tonsil and lymphoma tissues. IP: MERTK IP in HEK-293 cell lysate.

  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    ab271851 is the carrier-free version of ab52968. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
IP
Human
アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.

 

 
IP
Use at an assay dependent concentration.

 

 
特記事項
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.

 

 
IP
Use at an assay dependent concentration.

 

 
  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.

    • ターゲット情報

    • 機能

      In case of filovirus infection, seems to function as a cell entry factor.

    • 組織特異性

      Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.

    • 関連疾患

      Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.

    • 配列類似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • c MER antibody
        • c mer proto oncogene tyrosine kinase antibody
        • c-mer antibody
        • cMER antibody
        • cmer protooncogene tyrosine kinase antibody
        • Eyk antibody
        • MER antibody
        • MER receptor tyrosine kinase antibody
        • MERK antibody
        • MERPEN antibody
        • Mertk antibody
        • MERTK c-mer proto-oncogene tyrosine kinase antibody
        • MERTK_HUMAN antibody
        • MGC133349 antibody
        • nmf12 antibody
        • Nyk antibody
        • Proto oncogene tyrosine protein kinase MER antibody
        • Proto oncogene tyrosine protein kinase MER precursor antibody
        • Proto-oncogene c-Mer antibody
        • Receptor tyrosine kinase MerTK antibody
        • RP38 antibody
        • STK kinase antibody
        • Tyrosine-protein kinase Mer antibody
        see all

      画像

      • Immunoprecipitation - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)
        Immunoprecipitation - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)

        Lane 1: ab52968 (purified) at 1/20 immunoprecipitating MERTK in HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate.

        Lane 2 - PBS.

        For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
        Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52968).
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MERTK with purified ab52968 at 1/500.

        Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

        Negative control using PBS instead of primary antibody (Inset).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52968).
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labeling MERTK with unpurified ab52968 at a 1/50 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52968).
      • Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)
        Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab271851 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab271851 は論文での使用が確認できていません。

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