Anti-MERTK 抗体 [Y323] (ab52968)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y323] to MERTK
- Suitable for: WB, IP, IHC-P, IHC-Fr
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MERTK antibody [Y323]
MERTK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y323] to MERTK -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-P, IHC-Frmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human MERTK aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: A172, HEK-293 and K562 cell lysate. ild-type HAP1 whole cell lysate IHC-P: Human tonsil and lymphoma tissues. IP: MERTK IP in HEK-293 cell lysate. IHC-Fr: Human spleen tissue sections.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y323 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52968の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/2000. Detects a band of approximately 180 kDa.
For unpurified use at 1/500. |
IP |
1/20.
For unpurified use at 1/50. |
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IHC-P | (3) |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
Use at an assay dependent concentration.
|
特記事項 |
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WB
1/2000. Detects a band of approximately 180 kDa. For unpurified use at 1/500. |
IP
1/20. For unpurified use at 1/50. |
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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機能
In case of filovirus infection, seems to function as a cell entry factor. -
組織特異性
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
関連疾患
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10461 Human
- Omim: 604705 Human
- SwissProt: Q12866 Human
- Unigene: 306178 Human
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別名
- c MER antibody
- c mer proto oncogene tyrosine kinase antibody
- c-mer antibody
see all
画像
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All lanes : Anti-MERTK antibody [Y323] (ab52968) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MERTK knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.Lanes 1 - 2: Merged signal (red and green). Green - ab52968 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab52968 was shown to specifically react with MERTK in wild-type HAP1 cells as signal was lost in MERTK knockout cells. Wild-type and MERTK knockout samples were subjected to SDS-PAGE. The membrane was blocked with 0. Ab52968 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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ab52968 used at a 1/200 dilution, 18 hours at 4°C, 5% BSA in Tween PBS.
Positive cell line: A172
Negative cell lines: CV1 and HeLa.
Secondary used is a goat anti-rabbit polyclonal-HRP at 1/10000 dilution.
Blocked with 5% milk for 1 hour at RT.
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All lanes : Anti-MERTK antibody [Y323] (ab52968) at 1/2000 dilution (purified)
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution
Observed band size: 140-180 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
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Lane 1: ab52968 (purified) at 1/20 immunoprecipitating MERTK in HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 - PBS.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MERTK with purified ab52968 at 1/500.
Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.
Negative control using PBS instead of primary antibody (Inset).
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IHC image of MERTK staining in a section of frozen normal human spleen performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab52968, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Anti-MERTK antibody [Y323] (ab52968) at 1/1000 dilution (unpurified) + HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Observed band size: 180 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labeling MERTK with unpurified ab52968 at a 1/50 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (57)
ab52968 は 57 報の論文で使用されています。
- Liu Y et al. N-glycosylation stabilizes MerTK and promotes hepatocellular carcinoma tumor growth. Redox Biol 54:102366 (2022). PubMed: 35728303
- Tian H et al. Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration. iScience 25:105050 (2022). PubMed: 36185374
- Tang C et al. A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface. Commun Biol 4:161 (2021). PubMed: 33547385
- Gillen KM et al. QSM is an imaging biomarker for chronic glial activation in multiple sclerosis lesions. Ann Clin Transl Neurol 8:877-886 (2021). PubMed: 33704933
- Biswas P et al. Detection and validation of novel mutations in MERTK in a simplex case of retinal degeneration using WGS and hiPSC-RPEs model. Hum Mutat 42:189-199 (2021). PubMed: 33252167