Anti-MERTK 抗体 [EPR23248-259] - BSA and Azide free (ab272177)
![Western blot - Anti-MERTK antibody [EPR23248-259] - BSA and Azide free (ab272177)](https://www.abcam.co.jp/ps/products/272/ab272177/Images/ab272177-392896-anti-mertk-antibody-epr23248-259-western-blot-ovary-testis-human.jpg "Western blot - Anti-MERTK antibody [EPR23248-259] - BSA and Azide free (ab272177)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23248-259] to MERTK - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
製品の概要
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製品名
Anti-MERTK antibody [EPR23248-259] - BSA and Azide free
MERTK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23248-259] to MERTK - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HEK-293, HepG2, U-937, Human spleen, Human ovary, Human testis, Human liver, Mouse spleen, Mouse kidney and Rat spleen, Rat kidney lysates.
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特記事項
ab272177 is the carrier-free version of ab270448. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23248-259 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab272177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 110 kDa. |
ターゲット情報
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機能
In case of filovirus infection, seems to function as a cell entry factor. -
組織特異性
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
関連疾患
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10461 Human
- Entrez Gene: 17289 Mouse
- Entrez Gene: 65037 Rat
- Omim: 604705 Human
- SwissProt: Q12866 Human
- SwissProt: Q60805 Mouse
- SwissProt: P57097 Rat
- Unigene: 306178 Human
see all -
別名
- c MER antibody
- c mer proto oncogene tyrosine kinase antibody
- c-mer antibody
see all
画像
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Western blot - Anti-MERTK antibody [EPR23248-259] - BSA and Azide free (ab272177)All lanes : Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution
Lane 1 : Human ovary tissue lysate
Lane 2 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 110 kDa
Observed band size: 150, 210 kDa why is the actual band size different from the predicted?Exposure time:
Lane 1: 3 minutes
Lane 2: 70 secondsBlocking/Diluting buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270448).
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![Anti-MERTK antibody [EPR23248-259] - BSA and Azide free (ab272177)](https://www.abcam.co.jp/ps/products/272/ab272177/Images/ab272177-4-benefits-of-recombinant-antibodies.png)
Anti-MERTK antibody [EPR23248-259] - BSA and Azide free (ab272177)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
Certificate of Compliance
参考文献 (0)
ab272177 は論文での使用が確認できていません。
