Anti-MERTK 抗体 [EPR23248-259] (ab270448)
![Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448)](https://www.abcam.co.jp/ps/products/270/ab270448/Images/ab270448-392769-anti-mertk-antibody-epr23248-259-western-blot-ovary-testis-human.jpg "Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23248-259] to MERTK
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
製品の概要
-
製品名
Anti-MERTK antibody [EPR23248-259]
MERTK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23248-259] to MERTK -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
-
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- WB: HEK-293, HL-60, HepG2, and U-937 whole cell lysates. Human ovary, Human testis, Mouse spleen, Mouse kidney, Rat spleen, and Rat kidney tissue lysates.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23248-259 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Related Products
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| Flow Cyt |
Mouse
Human
|
| ICC |
Mouse
Human
|
| IHC-Fr |
Mouse
Rat
|
| IHC-P |
Rat
Human
|
| IP |
Mouse
Human
|
| WB |
Mouse
Rat
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 110 kDa.
|
| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 110 kDa. |
ターゲット情報
-
機能
In case of filovirus infection, seems to function as a cell entry factor. -
組織特異性
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
関連疾患
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 10461 Human
- Entrez Gene: 17289 Mouse
- Entrez Gene: 65037 Rat
- Omim: 604705 Human
- SwissProt: Q12866 Human
- SwissProt: Q60805 Mouse
- SwissProt: P57097 Rat
- Unigene: 306178 Human
see all -
別名
- c MER antibody
- c mer proto oncogene tyrosine kinase antibody
- c-mer antibody
see all
画像
-
Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448)All lanes : Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution
Lane 1 : Human ovary tissue lysate
Lane 2 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 110 kDa
Observed band size: 150, 210 kDa why is the actual band size different from the predicted?Exposure time:
Lane 1: 3 minutes
Lane 2: 70 secondsBlocking/Diluting buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
-
![Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448)](https://www.abcam.co.jp/ps/products/270/ab270448/Images/ab270448-386922-anti-mertk-antibody-epr23248259-western-blot-mouse-spleen-mouse-kidneyrat-spleen-rat-kidney-lysates.jpg)
Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448)All lanes : Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat spleen tissue lysate
Lane 4 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 110 kDa
Observed band size: 210/150 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Exposure time:
Lane 1: 70 seconds
Lanes 2-4: 3 minutes
-
![Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448)](https://www.abcam.co.jp/ps/products/270/ab270448/Images/ab270448-386917-anti-mertk-antibody-epr23248259-western-blot-hek293-hl60hepg2-u937-lysates.jpg)
Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448)All lanes : Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution
Lane 1 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 4 : U-937 (human histiocytic lymphoma monocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 110 kDa
Observed band size: 210/150 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: HL-60 (PMID:23474756)
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 48 seconds
-
![Anti-MERTK antibody [EPR23248-259] (ab270448)](https://www.abcam.co.jp/ps/products/270/ab270448/Images/ab270448-6-benefits-of-recombinant-antibodies.png)
Anti-MERTK antibody [EPR23248-259] (ab270448)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
Certificate of Compliance
参考文献 (0)
ab270448 は論文での使用が確認できていません。
