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  1. Link

    menin-antibody-ab2605.pdf

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Epigenetics and Nuclear Signaling Transcription Cancer susceptibility Tumor Suppressors
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Anti-Menin 抗体 (ab2605)

  • Datasheet
  • SDS
Reviews (1)Q&A (9)References (20)

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Western blot - Anti-Menin antibody - ChIP Grade (ab2605)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)
  • Immunoprecipitation - Anti-Menin antibody (ab2605)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)

Key features and details

  • Rabbit polyclonal to Menin
  • Suitable for: ICC/IF, ChIP, IHC-P, WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Menin antibody [EPR3986] (ab92443)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Menin antibody
    Menin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Menin
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, ChIP, IHC-P, WB, IPmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide (Human) - which represents a portion of the C-terminus of human MEN1.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 特記事項(精製)

    Affinity purified using the immunising peptideimmobilized on solid support.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors

関連製品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Recombinant Menin protein (ab114387)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Menin protein (ab114387)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2605の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
Use at an assay dependent concentration.
ChIP
Use at an assay dependent concentration.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/10000 - 1/25000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).Can be blocked with Recombinant Menin protein (ab114387).
IP
Use at 2-10 µg/mg of lysate.
特記事項
ICC/IF
Use at an assay dependent concentration.
ChIP
Use at an assay dependent concentration.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/10000 - 1/25000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).Can be blocked with Recombinant Menin protein (ab114387).
IP
Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能

    Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.
  • 組織特異性

    Ubiquitous.
  • 関連疾患

    Defects in MEN1 are the cause of familial multiple endocrine neoplasia type I (MEN1) [MIM:131100]. Autosomal dominant disorder characterized by tumors of the parathyroid glands, gastro-intestinal endocrine tissue, the anterior pituitary and other tissues. Cutaneous lesions and nervous-tissue tumors can exist. Prognosis in MEN1 patients is related to hormonal hypersecretion by tumors, such as hypergastrinemia causing severe peptic ulcer disease (Zollinger-Ellison syndrome, ZES), primary hyperparathyroidism, and acute forms of hyperinsulinemia.
    Defects in MEN1 are the cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors.
  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在

    Nucleus. Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation.
  • Target information above from: UniProt accession O00255 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 539431 Cow
    • Entrez Gene: 483758 Dog
    • Entrez Gene: 100722977 Guinea pig
    • Entrez Gene: 100050500 Horse
    • Entrez Gene: 4221 Human
    • Entrez Gene: 17283 Mouse
    • Entrez Gene: 100523533 Pig
    • Entrez Gene: 29417 Rat
    • Omim: 613733 Human
    • SwissProt: Q0P5I0 Cow
    • SwissProt: A2SXS5 Dog
    • SwissProt: O00255 Human
    • SwissProt: O88559 Mouse
    • SwissProt: Q9WVR8 Rat
    • Unigene: 423348 Human
    • Unigene: 12917 Mouse
    • Unigene: 453222 Mouse
    • Unigene: 6775 Rat
    see all
  • 別名

    • MEA 1 antibody
    • MEA1 antibody
    • MEN 1 antibody
    • Men1 antibody
    • MEN1_HUMAN antibody
    • Menin antibody
    • Multiple Endocrine Adenomatosis 1 antibody
    • Multiple Endocrine Neoplasia 1 antibody
    • SCG 2 antibody
    • SCG2 antibody
    • Suppressor Candidate Gene 2 antibody
    • Wermer syndrome antibody
    • ZES antibody
    • Zollinger Ellison Syndrome antibody
    see all

画像

  • Western blot - Anti-Menin antibody - ChIP Grade (ab2605)
    Western blot - Anti-Menin antibody - ChIP Grade (ab2605)
    All lanes : Anti-Menin antibody (ab2605) at 0.04 µg/ml

    Lane 1 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 68 kDa



    Samples in NETN lysis buffer.

    Exposure time: 30 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)

    Immunohistochemical analysis of human lung carcinoma tissue labeling Menin with ab2605 at 1 µg/ml. Detection: DAB.

  • Immunoprecipitation - Anti-Menin antibody (ab2605)
    Immunoprecipitation - Anti-Menin antibody (ab2605)

    Menin was imunnoprecipitated from 1.0 mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab2605 at 6 µg/ml. Western blot was perfomed from the immunoprecipitate using ab2506 at 0.04 µg/ml.

    Lane 1 and 2: 20% of ab2605 IP in HEK-293T whole cell lysate 

    Lane 3: Control IgG 

    Exposure time: 1 second

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)

    Immunohistochemical analysis of human breast carcinoma tissue labelling Menin with ab2605 at 1µg/ml. Detection: DAB.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (20)

ab2605 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2605 は 20 報の論文で使用されています。

  • Yoshiji S  et al. Alu-Mediated MEN1 Gene Deletion and Loss of Heterozygosity in a Patient with Multiple Endocrine Neoplasia Type 1. J Endocr Soc 4:bvaa051 (2020). PubMed: 32715270
  • Lines KE  et al. Genetic background influences tumour development in heterozygous Men1 knockout mice. Endocr Connect 9:426-437 (2020). PubMed: 32348957
  • Lines KE  et al. MiR-15a/miR-16-1 expression inversely correlates with cyclin D1 levels in Men1 pituitary NETs. J Endocrinol N/A:N/A (2018). PubMed: 30389902
  • Uraki S  et al. Atypical pituitary adenoma with MEN1 somatic mutation associated with abnormalities of DNA mismatch repair genes; MLH1 germline mutation and MSH6 somatic mutation. Endocr J 64:895-906 (2017). PubMed: 28701629
  • Shell J  et al. SomaticVHLMutation in a Patient With MEN1-Associated Metastatic Pancreatic Neuroendocrine Tumor Responding to Sunitinib Treatment: A Case Report. J Endocr Soc 1:1124-1134 (2017). PubMed: 29264567
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 10 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Menin antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hella cell)
Specification
Hella cell
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Jiang Hu

Verified customer

投稿 Jun 01 2007

Question

Thank you for sending me this email and give me a list of human ChIP primers as follow:
https://www.abcam.com/index.html?pageconfig=resource&rid=12281 Credit Note ID: 25650
I also asked the positive primers specific to menin ChIP. I think this will be more helpful if you can give to positive primers to menin ChIP instead of general list of primers.

Read More

Abcam community

Verified customer

Asked on Apr 12 2013

Answer


The following references may be useful to you for specific primers for positive and negative loci associated with Menin:

ab31902 and ab32963: Menin highly enriched in PCNA PRO 2, weakly enriched in PCNA PRO 1 according to PMID:18940868 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582630/?tool=pubmed

ab2605 in ChIP: http://cancerres.aacrjournals.org/content/72/1/165.full.pdf+html

Read More

Abcam Scientific Support

Answered on Apr 12 2013

Question



Thanks for the generous offer. It was for two antibodies including two vials of Ab21873. Note that the order dates from 28/9/2010 and as I pointed out in my previous E-mail, we did receive replacements with an older batch. All I can say is that if lot number GR21097-1 works for chromatin immunoprecipitation, we will be happy to pay for it.

Read More

Abcam community

Verified customer

Asked on Feb 27 2012

Answer

I have issued a free of charge replacement with the order number 1042072.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that typically our Abpromise guarantee holds for 6 months from when you receive the antibody,although I have made an exception in this case.Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on Feb 27 2012

Question

Thank you for the offer of a replacement vial. However, I suspect that the previous "complaint" you refer to came from us and Abcam kindly replaced the antibody with an earlier batch. I don't think we should expect more.



My question, which was simply prompted by an E-mail from Abcam, was whether there is now a more recent batch of ab21873 (presumably lot GR21097-1) and if so, would it be possible for us to test an aliquot in ChIP conditions before buying several vials.

Read More

Abcam community

Verified customer

Asked on Feb 24 2012

Answer

Thanks for your reply.

You are correct that lot GR21097-1 is a new lot we have in stock. We are not able to stock small sample aliqots of our antibodies unfortunately. However, since a vial of ab21873 you previously purchased is not working as expected,I can provide you with a free replacement vial of this new lot based on our Abpromise guarantee https://www.abcam.com/abpromise

If you would like to go ahead with the replacement offer, please just provide me with the PO number or Abcam order reference number associated with the purchase of ab21873, lot 937205 so I can process the replacement.

Read More

Abcam Scientific Support

Answered on Feb 24 2012

Question


As it happens, we have been having some issues with batch-to-batch
variation with an antibody against CBX7 (AB21873) that we use extensively
for chromatin immunoprecipitation (ChIP). Lot # 937204 worked very well
whereas Lot #937205 did not. We did manage to get what we believe may
have been the last aliquots of the good batch but are just about to run
out. If there have been any more recent batches, we would be very keen to
try some before restocking.

Read More

Abcam community

Verified customer

Asked on Feb 23 2012

Answer

Thank you for your enquiry. I am sorry to hear of the trouble you are having with lot 937205 in ChIP. I looked in our records and there was one other complaint of a similar nature regarding ChIP with this lot of ab21873. We do have a new lot in stock now (lot GR21097-1) and I am happy to offer a replacement for the faulty vial of ab21873.

Please pass along the PO number or Abcam order reference number associated with the purchase of ab21873, lot 937205 so I can process the replacement.

Read More

Abcam Scientific Support

Answered on Feb 23 2012

Question

Phone call requesting information regarding the region recognised by Anti-Menin antibody ab2605.

Read More

Abcam community

Verified customer

Asked on Jan 26 2012

Answer

Thank you for contacting us.

I have looked into whether the anti-Menin antibody (ab2605)would be suitable for the experiments you are hoping to perform. Unfortunately, the immunogen used to raise this antibody is directed against the C-terminal end of human Menin. Through epitope mapping it has been established that the antibody specifically recognises a region betweenresidue 575 and theC-terminus (residue 615) of Menin. This antibody would therefore not be suitable if you are hoping todetect the regionN-terminal of residue 575.

I have however been able to find two other antibodies which we have been raised against peptidesderived from the N-terminal sequence of Menin which may be suitable for you.Ab32963 and ab75923 are both rabbit polyclonals which have been used in Western blotting.A blocking peptide is also available for ab32963, the catalogue code for this is ab32962.

I hope this information has been of help. If you require any further information please do not hesitate to ask.

Read More

Abcam Scientific Support

Answered on Jan 26 2012

Question

The customer wans to use the antibody ab2605 on rat. He wants to know the detailed amino acids of the immunogenwhich represents a portion of the C-terminus of human MEN1. Looking forwards to hearing from you soon, Thank you dor your time,

Read More

Abcam community

Verified customer

Asked on May 30 2008

Answer

Thank you for your enquiry. The epitope recognized by ab2605 maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). Unfortunately, the exact sequence information is proprietary. I hope this information will still be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on May 30 2008

Question

Can you tell me the epitope region for this product?

Read More

Abcam community

Verified customer

Asked on Mar 03 2008

Answer

Thank you for your enquiry. The epitope recognized by this product maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). Unfortunately, the exact sequence information is proprietary. If you would like an alignment done to a certain species, I can have that analyzed.

Read More

Abcam Scientific Support

Answered on Mar 03 2008

Question

Last month we have bought two menin antibodies. One of them is ab2605, which was created with an immunogen which represents a portion of the C-terminus of the human menin. We would like to know between which aminoacid positions of the menin aminoacid sequence were used to create the immunogen.

Read More

Abcam community

Verified customer

Asked on Nov 03 2006

Answer

Thank for your patience. I have received a reply from the originator of ab2605. The epitope recognized by ab2605 maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). I hope this information helps. Please don't hesitate to contact us again if you have further questions.

Read More

Abcam Scientific Support

Answered on Nov 07 2006

Question

I am enquiring about your menin antiboday (ab2605). I was wondering want the concentration of the primary antibody was used to get such a clear band and what the secondary antibody was and at what concentration was it used. The mentioned reference in the data sheet does not mention the above antibody. Secondly, were the cell lysates used in the figure overexpressing menin or was it endogenous?

Read More

Abcam community

Verified customer

Asked on Feb 06 2006

Answer

Thank you for your enquiry and my apologies for the delay in replying to you, I have just received further information from the lab to answer your questions. We are not sure of the exact dilution used for the image on the datasheet, it is between 1/1000 - 1/10000 and I would recommend to try a range of dilution when you do the preliminary experiments to determine optimal conditions. The secondary is a standard HRP conjugated anti rabbit antibody. The reference on the datasheet was indeed incorrect, however I found the following references which are specific for this antibody: Milne TA et al. Menin and MLL cooperatively regulate expression of cyclin-dependent kinase inhibitors. Proc Natl Acad Sci U S A 102:749-54 (2005). PubMed: 15640349 Yokoyama A et al. The menin tumor suppressor protein is an essential oncogenic cofactor for MLL-associated leukemogenesis. Cell 123:207-18 (2005). PubMed: 16239140 Hughes CM et al. Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. Mol Cell 13:587-97 (2004). PubMed: 14992727 The cells used were not tranfected i.e the samples show endogenous levels of menin. Please do not hesitate to contact me if you require further information,

Read More

Abcam Scientific Support

Answered on Feb 15 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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