Key features and details
- Goat polyclonal to Melanophilin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab2716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 65 kDa.
1 hour primary incubation is recommended for this product.
|ICC/IF||Use a concentration of 0.5 µg/ml.|
機能Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.
関連疾患Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3) [MIM:609227]. GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations.
配列類似性Contains 1 FYVE-type zinc finger.
Contains 1 RabBD (Rab-binding) domain.
- Information by UniProt
- 2210418F23Rik antibody
- 5031433I09Rik antibody
- AW228792 antibody
All lanes : Anti-Melanophilin antibody (ab2716) at 0.5 µg/ml
Lane 1 : total melanocyte PNS (200ug per lane) of wild-type
Lane 2 : total melanocyte PNS (200ug per lane) of leaden (Mlph null) mice
Predicted band size: 65 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
ab2716 staining (3
µg/ml) of Jurkat lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab2716 は 1 報の論文で使用されています。
- Kudo M et al. Bifunctional effects of O-methylated flavones from Scutellaria baicalensis Georgi on melanocytes: Inhibition of melanin production and intracellular melanosome transport. PLoS One 12:e0171513 (2017). PubMed: 28182699