Western blot abreview for Anti-Melanoma antibody [HMB45]

Below Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (transfected HeLa)
Loading amount
20 µg
Specification
transfected HeLa
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/20
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Milk 5%

Secondary antibody

Name
Non-Abcam antibody was used: HRP conjugated anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 30, 35 kDa Non-specific: 10, 15 , 20 kDa

Additional data

Abcam response
Thank you for your feedback. This product has not yet been tested for use in Western blotting and it is possible does not function well in this application.

Dr. Guillaume Van Niel

Verified customer

投稿 Jul 18 2007

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